Schwartz R, Kniep B, Müthing J, Mühlradt P F
Int J Cancer. 1985 Nov 15;36(5):601-7. doi: 10.1002/ijc.2910360514.
A syngeneic tumor system in DBA/2 mice consisting of a methyl-cholanthrene-induced, weakly metastatic lymphoma, L5178YE (= Eb), its spontaneous strongly metastatic variant, L5178YES (= ESb), and an unrelated, methylcholanthrene-induced, metastasizing tumor, MDAY-D2, were used to study the relationship between metastatic behavior and composition of GSLs. The D-1-14C galactose and D-1-14C glucosamine-labelled neutral GSL and gangliosides of these tumor cells, and additionally ConA-stimulated spleen T cells from normal mice, were analysed by thin-layer chromatography. Unlabelled GSLs of the tumors were also characterized by (HPLC) after perbenzoylation. Results obtained with the radioactively labelled GSLs correlated with those of the HPLC analysis of unlabelled GSLs. All tumors contained neutral GSL of the ganglio-series. Weakly metastatic tumor Eb showed neutral GSL patterns comparable to those from ConA-stimulated spleen cells, whereas strongly metastatic tumors ESb and MDAY-D2 had an enhanced expression of lactosylceramide. Gangliosides of metastatic ESb and MDAY-D2 had a higher degree of polarity than those of weakly metastatic Eb. Eb cells expressed primarily GM1. Metastasizing ESb and MDAY-D2 had significantly higher amounts of GM3, GM2 and GD1a. An unusual ganglioside, IV3GalNAc-GM1, was found in MDAY-D2 cells and ConA blasts. When the extent of label was compared in neutral GSLs and gangliosides, metastasizing ESb and MDAY-D2 were more heavily labelled in the ganglioside fraction (62%, 58%) than Eb (39%). ESb and MDAY-D2 also contained larger amounts of gangliosides than Eb.
在DBA/2小鼠中构建了一种同基因肿瘤系统，该系统由甲基胆蒽诱导的低转移性淋巴瘤L5178YE（=Eb）、其自发产生的高转移性变体L5178YES（=ESb）以及一种不相关的、甲基胆蒽诱导的转移性肿瘤MDAY-D2组成，用于研究转移行为与糖鞘脂（GSLs）组成之间的关系。通过薄层色谱分析了这些肿瘤细胞的D-1-14C半乳糖和D-1-14C葡糖胺标记的中性GSL和神经节苷脂，此外还分析了正常小鼠经刀豆蛋白A刺激的脾脏T细胞。肿瘤的未标记GSL在过苯甲酰化后也通过高效液相色谱（HPLC）进行了表征。放射性标记GSL的结果与未标记GSL的HPLC分析结果相关。所有肿瘤均含有神经节系列的中性GSL。低转移性肿瘤Eb显示出与刀豆蛋白A刺激的脾细胞相当的中性GSL模式，而高转移性肿瘤ESb和MDAY-D2的乳糖基神经酰胺表达增强。转移性ESb和MDAY-D2的神经节苷脂极性程度高于低转移性Eb的神经节苷脂。Eb细胞主要表达GM1。转移性ESb和MDAY-D2的GM3、GM2和GD1a含量显著更高。在MDAY-D2细胞和刀豆蛋白A刺激的母细胞中发现了一种不寻常的神经节苷脂IV3GalNAc-GM1。当比较中性GSL和神经节苷脂中的标记程度时，转移性ESb和MDAY-D2在神经节苷脂部分的标记更重（分别为62%、58%），而Eb为39%。ESb和MDAY-D2含有的神经节苷脂也比Eb多。
