Hara S, Ishiguro S, Mizuno K
Invest Ophthalmol Vis Sci. 1985 Nov;26(11):1631-4.
The rabbit corneal endothelium phagocytized polystyrene spheres 0.5 micron in diameter. After phagocytizing spheres, the endothelium degenerated, and lost from the Descemet's membrane. Lysosomal enzyme activities of the endothelium-Descemet's membrane complex, such as acid phosphatase, beta-glucuronidase and N-acetyl-beta-D-glucosaminidase, were assayed and the total activities per microgram DNA were almost constant. The unsedimentable activities in the complex, however, increased by phagocytosis of polystyrene spheres, which indicated an extralysosomal release of lysosomal enzymes. Released lysosomal enzymes probably would have accounted for the degeneration of the corneal endothelium.
兔角膜内皮细胞吞噬了直径为0.5微米的聚苯乙烯球体。吞噬球体后，内皮细胞发生退化，并从Descemet膜上脱落。对内皮-Descemet膜复合物的溶酶体酶活性，如酸性磷酸酶、β-葡萄糖醛酸酶和N-乙酰-β-D-氨基葡萄糖苷酶进行了测定，每微克DNA的总活性几乎保持恒定。然而，复合物中不可沉淀的活性因聚苯乙烯球体的吞噬作用而增加，这表明溶酶体酶发生了溶酶体外释放。释放的溶酶体酶可能是角膜内皮细胞退化的原因。
