Hirsch M, Faure J P, Marquet O, Payrau P
Arch Ophtalmol Rev Gen Ophtalmol. 1975 Mar;35(3):269-78.
The corneal cells are destroyed within a 10 mm diameter circular belt by liquid nitrogen application in vivo. The endothelial regeneration is followed from day to day using endothelial flat preparation technic, and is compared to the corneal thickness measurement and to the estimation of transparency. From the 5th day, Descemet's membrane is entirely covered by the regenerated endothelium; from this time, both thickness and transparency of the stroma become normal. Therefore, in spite of the persitence of morphologic abnormalities, the regenerating endothelial cells preserve or obtain again very rapidly their specific function of a barrier against hydration of the stroma.
通过在体内应用液氮，在直径10毫米的圆形区域内破坏角膜细胞。采用内皮平铺制备技术逐日跟踪内皮再生情况，并与角膜厚度测量及透明度评估结果进行比较。从第5天起，后弹力层完全被再生的内皮覆盖；从此时起，基质的厚度和透明度均恢复正常。因此，尽管存在形态学异常，但再生的内皮细胞能非常迅速地保留或再次获得其作为基质水化屏障的特定功能。
