Hu Jialin, Guo Lingling, Liu Liqiang, Xu Liguang, Kuang Hua, Xu Chuanlai, Xu Xinxin
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, PR China.
International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, PR China; State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu, PR China.
Food Chem. 2025 Oct 30;490:145188. doi: 10.1016/j.foodchem.2025.145188. Epub 2025 Jun 16.
Since allergic reaction triggered by sesame 2S protein can be life-threatening, a sensitive and rapid method to detect sesame 2S protein in foods is urgent needed. In this paper, based on the extraction and purification of sesame 2S protein, monoclonal antibodies against sesame 2S protein were prepared. The optimal antibody pair 7C12C2 was screened according to the performance of lateral flow immunochromatographic analysis (LFIA) strips. The calculated limits of detection (cLODs) of the strips for the detection of sesame 2S protein in milk, biscuits and bread were 14.70 ng/mL, 5.31 and 5.85 μg/kg, respectively. Notably, the results and recovery rates of the strips for spiked samples were in good agreement with those of high-performance liquid chromatography. The testing of positive samples further confirmed the reliability of LFIA strips. Collectively, our proposed strip can be used for the rapid, sensitive and reliable determination of sesame 2S protein in foods.
由于芝麻2S蛋白引发的过敏反应可能危及生命，因此迫切需要一种灵敏、快速的方法来检测食品中的芝麻2S蛋白。本文在芝麻2S蛋白提取和纯化的基础上，制备了抗芝麻2S蛋白的单克隆抗体。根据侧向流免疫层析分析（LFIA）试纸条的性能筛选出最佳抗体对7C12C2。该试纸条检测牛奶、饼干和面包中芝麻2S蛋白的计算检出限（cLODs）分别为14.70 ng/mL、5.31和5.85 μg/kg。值得注意的是，加标样品试纸条的检测结果和回收率与高效液相色谱法的结果高度一致。阳性样品检测进一步证实了LFIA试纸条的可靠性。总体而言，我们所提出的试纸条可用于快速、灵敏且可靠地测定食品中的芝麻2S蛋白。
