Liu Minxuan, Li Shuxian, Chen Yuyang, Li Jiaxi, Zhang Fuyuan, Wang Xianghong
College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, China.
Food Chem. 2025 Oct 15;489:144972. doi: 10.1016/j.foodchem.2025.144972. Epub 2025 May 29.
Florfenicol (FF) and its primary metabolite, florfenicol amine (FFA), have emerged as significant concerns in food safety in recent years. In this study, a monoclonal antibody specific to both FF and FFA was developed using hybridoma screening. We developed a dual-readout fluorescent lateral flow immunochromatographic assay (LFIA) for the rapid detection of FF and FFA. This LFIA employs chromogenic properties of CoO nanoparticles (NPs) and integrates the inner filter effect (IFE) between CoO NPs and gold nanoclusters (AuNCs), enabling both colorimetric and fluorescent readouts. The fluorescence "turn-off" mode achieved limits of detection (LOD) of 7.04 ng/mL for FF and 6.19 ng/mL for FFA. With the integration of a portable sensing device, the "turn-on" mode further improved the LOD to 3.73 ng/mL for FF and 4.06 ng/mL for FFA. The dual-readout LFIA provided a practical tool for detecting FF and FFA in real samples, offering a promising strategy for antibiotic detection.
氟苯尼考（FF）及其主要代谢物氟苯尼考胺（FFA）近年来已成为食品安全方面的重大关注点。在本研究中，通过杂交瘤筛选开发了一种对FF和FFA均具有特异性的单克隆抗体。我们开发了一种双读数荧光侧向流动免疫色谱分析方法（LFIA），用于快速检测FF和FFA。该LFIA利用了氧化钴纳米颗粒（NPs）的显色特性，并整合了氧化钴纳米颗粒与金纳米团簇（AuNCs）之间的内滤光效应（IFE），实现了比色和荧光读数。荧光“关闭”模式下，FF的检测限（LOD）为7.04 ng/mL，FFA的检测限为6.19 ng/mL。通过集成便携式传感设备，“开启”模式进一步将FF的LOD提高到3.73 ng/mL，FFA的LOD提高到4.06 ng/mL。这种双读数LFIA为检测实际样品中的FF和FFA提供了一种实用工具，为抗生素检测提供了一种有前景的策略。
