Maurastoni Marlonni, Rotenberg Dorith, Mishra Ruchir, Bonning Bryony C, Whitfield Anna E
Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, United States of America.
Department of Entomology and Nematology, University of Florida, Gainesville, Florida, United States of America.
PLoS One. 2025 Jun 24;20(6):e0326260. doi: 10.1371/journal.pone.0326260. eCollection 2025.
We investigated the protein composition of the brush border membrane of larval Frankliniella occidentalis (western flower thrips), an agriculturally significant crop pest and vector of plant pathogens. We developed a protocol for purifying brush border membrane vesicles (BBMVs) from first-instar larvae (L1) bodies and identified their protein composition by LC-MS/MS. From 2544 proteins identified, 469 were predicted to be secreted and part of the inner and outer plasma membrane leaflet using cell-localization prediction tools and homology with reviewed proteins in the UniProt database. Comparison to thrips tissue-specific proteomes revealed that 371 and 263 of the identified BBMV plasma membrane and secreted proteins matched the larval gut and adult salivary glands, respectively. Annotations of most of the proteins inferred 'catalytic activity' (56.3%) and 'binding' (49.6%), with an overrepresentation of proteins involved in protein digestion, specifically serine proteases, lipid transport, and ATPase activity. Bioinformatic-enabled comparisons to thrips tissue-specific proteomes and transcriptomes enabled us to predict the secretome and the plasma membrane proteins of larval thrips' gut epithelial cells, providing new targets for thrips control.
我们研究了西花蓟马幼虫刷状缘膜的蛋白质组成，西花蓟马是一种对农业具有重要意义的作物害虫和植物病原体传播媒介。我们开发了一种从一龄幼虫（L1）体内纯化刷状缘膜囊泡（BBMVs）的方法，并通过液相色谱-串联质谱（LC-MS/MS）鉴定了它们的蛋白质组成。在鉴定出的2544种蛋白质中，使用细胞定位预测工具并与UniProt数据库中经过审核的蛋白质进行同源性比较，预测有469种蛋白质是分泌型的，并且是内外质膜小叶的一部分。与蓟马组织特异性蛋白质组的比较表明，鉴定出的BBMV质膜蛋白和分泌蛋白中，分别有371种和263种与幼虫肠道和成虫唾液腺相匹配。大多数蛋白质的注释推断为“催化活性”（56.3%）和“结合”（49.6%），其中参与蛋白质消化的蛋白质，特别是丝氨酸蛋白酶、脂质转运和ATP酶活性的蛋白质占比过高。通过生物信息学与蓟马组织特异性蛋白质组和转录组进行比较，使我们能够预测幼虫蓟马肠道上皮细胞的分泌组和质膜蛋白，为蓟马防治提供了新的靶点。
