León-Campos Maria I, Claudio-Rizo Jesús A, Becerra-Rodriguez Juan J, Espindola-Serna Laura, Cano-Salazar Lucía F, Rodríguez-Fuentes Nayeli, Betancourt-Galindo Rebeca
Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Ing. J. Cárdenas Valdez S/N, República, 25280 Saltillo, Coahuila, Mexico.
Ingeniería en Biotecnología, Universidad Politécnica de Pénjamo, Carretera Irapuato, A La Piedad Km 44, 36900 El Derramadero, Guanajuato, Mexico.
Biomed Mater. 2025 Jul 2;20(4). doi: 10.1088/1748-605X/ade7e2.
Aloe vera is widely recognized for its healing, anti-inflammatory, moisturizing, and antibacterial properties, which are attributed to its rich biochemical composition, including polysaccharides, proteins, vitamins, and anthraquinones such as aloin and emodin. This study investigates the encapsulation of Aloe vera in collagen-polyurethane hydrogels to create a novel bioactive formulation for wound healing. Hydrogels with Aloe vera content ranging from 20-60 wt.% were prepared, forming scaffolds with a fibrillar-granular morphology and semi-crystalline surface. Encapsulation of Aloe vera resulted in semi-interpenetrating polymer networks (semi-IPNs), with the semi-interpenetration rate increasing as Aloe vera content rose. The formulation containing 60 wt.% Aloe vera significantly enhanced the superabsorbent capacity (2850 ± 210%) and crosslinking degree (38 ± 3%) of the matrix while reducing the loss factor (2.5 ± 0.4), resulting in a highly viscous gel ideal for moisture retention and uniform wound coverage. These hydrogels exhibited resistance to biodegradation in the presence of pepsin at skin pH (5.5) and demonstrated notable antibacterial activity, inhibiting(78%) and(57%) growth compared to 40 ppm gentamicin. Additionally, they facilitated a gradual release of ketorolac (up to 65% at pH 5.5 with 20 wt.% Aloe vera). The hydrogels were non-hemolytic and promoted the metabolic activity of monocytes and fibroblasts, enhancing cellular growth. Furthermore, the composition induced no inflammatory response but significantly boosted the secretion of the anti-inflammatory cytokine TGF-1, potentially enhancing wound healing.
芦荟因其愈合、抗炎、保湿和抗菌特性而被广泛认可，这些特性归因于其丰富的生化成分，包括多糖、蛋白质、维生素以及芦荟素和大黄素等蒽醌类化合物。本研究调查了将芦荟封装在胶原蛋白 - 聚氨酯水凝胶中，以创建一种用于伤口愈合的新型生物活性制剂。制备了芦荟含量在20 - 60 wt.%范围内的水凝胶，形成具有纤维状 - 颗粒状形态和半结晶表面的支架。芦荟的封装导致形成半互穿聚合物网络（semi - IPNs），随着芦荟含量的增加，半互穿率也增加。含有60 wt.%芦荟的制剂显著提高了基质的高吸水性（2850 ± 210%）和交联度（38 ± 3%），同时降低了损耗因子（2.5 ± 0.4），从而形成了一种非常适合保湿和均匀覆盖伤口的高粘性凝胶。这些水凝胶在皮肤pH值（5.5）下存在胃蛋白酶时表现出抗生物降解性，并具有显著的抗菌活性，与40 ppm庆大霉素相比，抑制（78%）和（57%）的生长。此外，它们促进了酮咯酸的逐渐释放（在pH 5.5且含有20 wt.%芦荟时高达65%）。这些水凝胶无溶血作用，并促进单核细胞和成纤维细胞的代谢活性，增强细胞生长。此外，该组合物未引发炎症反应，但显著促进了抗炎细胞因子TGF - 1的分泌，可能增强伤口愈合。
