Chen Zhipeng, Guo Jian, Chen Wenyi, Meng Yuan, Li Daxiao, Zhou Junhui, Wang Zhongjue
Department of Ophthalmology and Otorhinolaryngology，the Second People's Hospital of Longgang District，Shenzhen，518112，China.
Department of Pathology，the Second People's Hospital of Longgang District.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2025 Jul;39(7):617-623. doi: 10.13201/j.issn.2096-7993.2025.07.004.
To identify the key epithelial cell characteristics that can accurately diagnose eosinophilic chronic sinusitis with nasal polyps（ECRSwNP） through nasal brush sampling and comparing with the pathological results of nasal polyp tissue sections. Ninety-one patients underwent surgery in the Ophthalmology and ENT Department of the Second People's Hospital of Longgang District, Shenzhen, from January 2022 to July 2024 were selected. The cohort comprised 58 males and 33 females（mean age: 41.4 years; range: 12.0-71.0）. The clinical characteristics of the patients, including gender, age, disease duration, smoking and drinking history, asthma history, subjective symptoms, sinus CT, and nasal endoscopy scores, were recorded. Nasal brush sampling of nasal polyps and inferior turbinate mucosa was performed before surgery to obtain cytological specimens, and nasal polyp tissues were collected during surgery. The demographic and clinical characteristics of patients with eosinophilic and non-eosinophilic nasal polyps were compared, as well as the relationship between nasal brush cytology of nasal polyps and inferior turbinate and nasal polyp histopathology. Statistical analysis was performed using SPSS 23.0 software. Among the 91 patients, no significant differences were observed between ECRSwNP and NECRSwNP patients in terms of age, gender, smoking status, alcohol consumption, and disease duration. The nasal brush cell population in ECRSwNP patients was more likely to contain eosinophils（<0.001） and less likely to contain lymphocytes and plasma cells（<0.001）. Additionally, the ciliated cells in ECRSwNP patients exhibited larger widths（=0.036）, shorter cilium lengths（<0.001）, and more disordered arrangements（<0.001） compared to NECRSwNP patients. In nasal brush cells from the inferior turbinate, ECRSwNP patients also showed shorter cilium lengths（<0.001） and shorter cilia（=0.024） compared to NECRSwNP patients. There are significant differences in obtaining epithelial cytological information from nasal polyps or inferior turbinates through nasal brush sampling between ECRSwNP and NECRSwNP patients.
通过鼻刷采样识别可准确诊断嗜酸性粒细胞性慢性鼻窦炎伴鼻息肉（ECRSwNP）的关键上皮细胞特征，并与鼻息肉组织切片的病理结果进行比较。选取2022年1月至2024年7月在深圳市龙岗区第二人民医院眼科和耳鼻喉科接受手术的91例患者。该队列包括58名男性和33名女性（平均年龄：41.4岁；范围：12.0 - 71.0岁）。记录患者的临床特征，包括性别、年龄、病程、吸烟和饮酒史、哮喘史、主观症状、鼻窦CT和鼻内镜评分。术前对鼻息肉和下鼻甲黏膜进行鼻刷采样以获取细胞学标本，并在手术期间收集鼻息肉组织。比较嗜酸性粒细胞性和非嗜酸性粒细胞性鼻息肉患者的人口统计学和临床特征，以及鼻息肉和下鼻甲的鼻刷细胞学与鼻息肉组织病理学之间的关系。使用SPSS 23.0软件进行统计分析。在91例患者中，ECRSwNP患者和非嗜酸性粒细胞性慢性鼻窦炎伴鼻息肉（NECRSwNP）患者在年龄、性别、吸烟状况、饮酒和病程方面未观察到显著差异。ECRSwNP患者的鼻刷细胞群体更可能含有嗜酸性粒细胞（<0.001），而含有淋巴细胞和浆细胞的可能性较小（<0.001）。此外，与NECRSwNP患者相比，ECRSwNP患者的纤毛细胞宽度更大（=0.036），纤毛长度更短（<0.001）且排列更紊乱（<0.001）。在下鼻甲鼻刷细胞中，与NECRSwNP患者相比，ECRSwNP患者的纤毛长度也更短（<0.001）且纤毛更短（=0.024）。ECRSwNP患者和NECRSwNP患者通过鼻刷采样从鼻息肉或下鼻甲获取上皮细胞学信息存在显著差异。
