通过液相色谱和气相色谱-质谱联用技术对磺胺二甲嘧啶代谢物进行鉴定和定量分析。

Identification and quantitation of sulfamethazine metabolites by liquid chromatography and gas chromatography-mass spectrometry.

作者信息

Paulson G D, Mitchell A D, Zaylskie R G

出版信息

J Assoc Off Anal Chem. 1985 Sep-Oct;68(5):1000-6.

Abstract

Methods for the identification and quantitation of carbon-14 labeled sulfamethazine [4-amino-N-(4,6-dimethyl-2-pyrimidinyl)benzenesulfonamide], N4-acetylsulfamethazine, the N4-glucose conjugate of sulfamethazine, and desaminosulfamethazine in swine tissue are described. Tissues are ground and extracted with methanol, and the 14C-labeled compounds are purified by XAD-2 column chromatography and C-18 reverse phase liquid chromatography (LC) and the 14C-labeled compounds are then methylated and identified by gas chromatography-mass spectrometry analysis. Quantitation is accomplished by measuring the amount of 14C-activity that cochromatographs (C-18 reverse phase LC) with reference compounds.

摘要

描述了猪组织中碳-14标记的磺胺二甲嘧啶[4-氨基-N-(4,6-二甲基-2-嘧啶基)苯磺酰胺]、N4-乙酰磺胺二甲嘧啶、磺胺二甲嘧啶的N4-葡萄糖共轭物以及脱氨基磺胺二甲嘧啶的鉴定和定量方法。将组织研磨并用甲醇提取，14C标记的化合物通过XAD-2柱色谱和C-18反相液相色谱(LC)纯化，然后将14C标记的化合物甲基化并通过气相色谱-质谱分析进行鉴定。通过测量与参考化合物共色谱( C-18反相LC)的14C活性量来完成定量。

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