Moats W A
J Assoc Off Anal Chem. 1985 Sep-Oct;68(5):980-4.
Tylosin, an antibiotic developed specifically for agricultural use, and erythromycin are the main macrolide antibiotics used in animal production. Two-dimensional thin layer chromatography has been used for detection of tylosin in poultry meat, eggs, and milk and for erythromycin in poultry meat. Detection limits reported are, for tylosin, 0.1 ppm in poultry meat, 0.05 ppm in egg, and 0.01 ppm in milk, and for erythromycin, 0.25 ppm in poultry meat. Liquid chromatography (LC) has also been used for determination of tylosin in milk, blood, and tissues of animals. Samples (milk, blood serum, or tissue homogenates in water or pH 2.2 buffer) were deproteinized with acetonitrile, tylosin was partitioned into methylene chloride, and the extracts were concentrated and dissolved in acetonitrile. Chromatography was done on a reverse phase end-capped C18 column using 0.002-0.005 M ammonium dihydrogen phosphate-acetonitrile-methanol (10 + 60 + 30-5 + 80 + 15). Solvent composition was varied with the type of sample analyzed. The method will detect 0.1 ppm tylosin in tissues and less in milk and blood serum. The LC method was more sensitive than microbiological assays for detection of tylosin in tissues of treated swine; recoveries of tylosin by the LC method were frequently several-fold higher.
泰乐菌素是一种专门为农业用途开发的抗生素，与红霉素是动物生产中使用的主要大环内酯类抗生素。二维薄层色谱法已用于检测禽肉、蛋类和牛奶中的泰乐菌素以及禽肉中的红霉素。报道的检测限为：禽肉中泰乐菌素为0.1 ppm，蛋类中为0.05 ppm，牛奶中为0.01 ppm；禽肉中红霉素为0.25 ppm。液相色谱法（LC）也已用于测定动物的牛奶、血液和组织中的泰乐菌素。样品（牛奶、血清或水或pH 2.2缓冲液中的组织匀浆）用乙腈进行脱蛋白处理，泰乐菌素分配到二氯甲烷中，提取物浓缩后溶解于乙腈。在反相封端C18柱上进行色谱分析，使用0.002 - 0.005 M磷酸二氢铵 - 乙腈 - 甲醇（10 + 60 + 30 - 5 + 80 + 15）。溶剂组成根据分析的样品类型而变化。该方法可检测组织中0.1 ppm的泰乐菌素，牛奶和血清中的含量更低。在检测经处理猪的组织中的泰乐菌素时，液相色谱法比微生物测定法更灵敏；液相色谱法对泰乐菌素的回收率通常高出几倍。
