Horii S, Momma C, Miyahara K, Maruyama T, Matsumoto M
Tokyo Metropolitan Research Laboratory of Public Health, Department of Food Hygiene and Nutrition, Japan.
J Assoc Off Anal Chem. 1990 Nov-Dec;73(6):990-2.
Sulfonamides are widely used as a feed additive in animal production in Japan. The present paper is a determination of 3 sulfonamides: sulfamethazine (SMZ), sulfamonomethoxine [SMX, 4-amino-N-(3-methoxypyrazinyl)-benzenesulfonamide], and sulfadimethoxine (SDX) in animal tissue and egg by liquid chromatography (LC). Tissues were extracted with acetonitrile and fat was removed by liquid/liquid partition. The sulfonamides were purified by an ODS cartridge column; then each compound was separated by an ODS LC column and detected at 268 nm. Quantification levels were 0.02 ppm for SMZ and SMX, and 0.04 ppm for SDX; detection limits were 0.01 ppm for SMZ and SMX, and 0.02 ppm for SDX. Calibration curves were linear between 2 and 40 ng for SMZ and SMX, and between 4 and 80 ng for SDX. Recoveries from muscle and egg samples spiked with 1-2 micrograms/10 g were 81-98%.
在日本，磺胺类药物被广泛用作动物生产中的饲料添加剂。本文采用液相色谱法（LC）测定动物组织和蛋类中的3种磺胺类药物：磺胺二甲嘧啶（SMZ）、磺胺间甲氧嘧啶[SMX，4-氨基-N-（3-甲氧基吡嗪基）苯磺酰胺]和磺胺二甲氧嘧啶（SDX）。用乙腈提取组织，通过液液分配去除脂肪。磺胺类药物用ODS柱净化；然后用ODS液相色谱柱分离各化合物，并在268nm处检测。定量限为SMZ和SMX为0.02ppm，SDX为0.04ppm；检测限为SMZ和SMX为0.01ppm，SDX为0.02ppm。SMZ和SMX的校准曲线在2至40ng之间呈线性，SDX在4至80ng之间呈线性。在添加了1-2微克/10克的肌肉和蛋类样品中的回收率为81-98%。
