We present a genome assembly from a female specimen of (Dalmatian wall lizard; Chordata; Lepidosauria; Squamata; Lacertidae). The assembly contains two haplotypes with total lengths of 1,574.91 megabases and 1,437.04 megabases. Most of haplotype 1 (94.94%) is scaffolded into 20 chromosomal pseudomolecules, including the W and Z sex chromosomes, while most of haplotype 2 (97.63%) is scaffolded into 18 chromosomal pseudomolecules. The mitochondrial genome has also been assembled, with a length of 18.2 kilobases.