Aishbani Sumayah J, Al-Griw Huda H, Al-Sharif Jihan A, Karim Elfurgani S, Farag Samira A, Ahmed Mohamed O, Abouzeed Yousef M
Department of Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Department Food Health Control, Faculty of Veterinary Medicine, University of Tripoli, Tripoli, Libya.
Open Vet J. 2025 May;15(5):1941-1946. doi: 10.5455/OVJ.2025.v15.i5.8. Epub 2025 May 31.
Nontyphoidal Salmonella (NTS) serovars remain a potential hazard to human and animal health. Food animals with infections can either be cured or remain asymptomatic carriers, serving as a potential source of infection to humans and other healthy animals. may also be a reservoir of resistance determinants that can be transferred to other commensal organisms, particularly in the gastrointestinal tract.
This article addresses the prevalence of serovars in important food animals and their environment. Antibiotics susceptibility profiling of serovars against important antibiotics used in human and animal medicines was also investigated.
In total, 125 fecal samples were collected from food animals ( = 100 fecal samples) and from the environment ( = 25). Salmonellae were isolated using standard laboratory procedures, and typical isolates were further confirmed using laboratory biomedical testing and the automated microbial identification system VITEK 2. Confirmed isolates were further analyzed for their susceptibility to antimicrobial agents.
A total of 10.4% (13/125) of samples were found positive for by conventional isolation, biochemical identification methods, and VITEK 2 system. The prevalence of spp. in cattle feces was 8.9% (4/45), whereas the prevalence in poultry samples was 14.5% (8/55). Only one sample from soil was found positive for representing 6.7% (1/25). Serologically, only five isolates were further confirmed and typed into three serovars: ( = 2), ( = 1), and ( = 2). All isolates displayed minimum inhibitory concentrations values above the susceptibility breakpoint for ampicillin, piperacillin, and second-generation cephalosporins but showed reduced susceptibility to third-generation cephalosporins. All the isolates also displayed different levels of resistance to ciprofloxacin and levofloxacin. Resistance to the aminoglycosides (amikacin, tobramycin, and gentamicin) was also rerecorded.
The current study demonstrated that the high resistance rates to fluoroquinolones can compromise important therapeutic options against salmonellosis posing also public health concerns.
非伤寒沙门氏菌血清型仍然对人类和动物健康构成潜在危害。感染的食用动物要么被治愈，要么成为无症状携带者，成为人类和其他健康动物的潜在感染源。它们也可能是耐药决定因素的储存库，这些耐药决定因素可转移到其他共生生物，尤其是在胃肠道中。
本文探讨重要食用动物及其环境中非伤寒沙门氏菌血清型的流行情况。还研究了非伤寒沙门氏菌血清型对人用和兽用重要抗生素的药敏谱。
总共从食用动物（n = 100份粪便样本）和环境（n = 25份）中收集了125份粪便样本。使用标准实验室程序分离沙门氏菌，并使用实验室生物医学检测和自动化微生物鉴定系统VITEK 2进一步确认典型分离株。对确认的非伤寒沙门氏菌分离株进一步分析其对抗菌剂的敏感性。
通过传统分离、生化鉴定方法和VITEK 2系统，共发现10.4%（13/125）的样本非伤寒沙门氏菌呈阳性。牛粪便中非伤寒沙门氏菌的流行率为8.9%（4/45），而家禽样本中的流行率为14.5%（8/55）。仅一份土壤样本中非伤寒沙门氏菌呈阳性，占6.7%（1/25）。血清学上，仅五株非伤寒沙门氏菌分离株得到进一步确认，并分为三个血清型：肠炎沙门氏菌（n = 2）、鼠伤寒沙门氏菌（n = 1）和都柏林沙门氏菌（n = 2）。所有非伤寒沙门氏菌分离株对氨苄西林、哌拉西林和第二代头孢菌素的最低抑菌浓度值均高于敏感断点，但对第三代头孢菌素的敏感性降低。所有分离株对环丙沙星和左氧氟沙星也表现出不同程度的耐药性。对氨基糖苷类（阿米卡星、妥布霉素和庆大霉素）的耐药性也有记录。
当前研究表明，对氟喹诺酮类药物的高耐药率可能会影响针对沙门氏菌病的重要治疗选择，也引起了公共卫生方面的关注。
