Gao Ziyu, Criscuolo Valeria, Formiggini Fabio, De Martino Ilaria, Perna Daniela, Siciliano Velia, Santoro Francesca
Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia, Naples, Italy.
Institute of Biological Information Processing - Bioelectronics, IBI-3, Forschungszentrum Jülich, Jülich, Germany.
Nanoscale. 2025 Jul 10;17(27):16493-16502. doi: 10.1039/d4nr04659e.
Morphic biomaterials have been widely utilized as cell instructive platforms to regulate biological functions of cells and tissues. Here, light can be exploited to tune the mechanical response of these materials (, azobenzene-based polymers) to reshape and induce dimensionality changes (, from 2D to 3D geometries). In fact, 3D systems better replicate the cell-tissue environment and its continuous conformational changes. In this work, we present a 3D pillar-based platform capable of undergoing reshaping and dislocation. These properties can be precisely controlled at the micro-nano scale through characteristic light irradiation parameters such as intensity and exposure time. In this work, light-driven deformation resulting in elongation, polarization, and substructure generation (, nanobump-like structures) was achieved using low-intensity (5%) light exposure. Furthermore, deformation in sliding and rotation was obtained with high laser intensity (20-100%, % of 10 mW input), based on which, a photo-induced deformation model was proposed for azobenzene-based polymers. Additionally, the biocompatibility of the azopolymer-based pillars was proven as well as their potential to locally reshape and induce real-time cell response.
形态生物材料已被广泛用作细胞指导平台，以调节细胞和组织的生物学功能。在此，光可用于调节这些材料（基于偶氮苯的聚合物）的机械响应，以重塑并诱导尺寸变化（例如从二维几何形状变为三维几何形状）。事实上，三维系统能更好地复制细胞 - 组织环境及其持续的构象变化。在这项工作中，我们展示了一种能够进行重塑和位错的基于三维支柱的平台。这些特性可以通过诸如强度和曝光时间等特征光照射参数在微纳米尺度上精确控制。在这项工作中，使用低强度（5%）光照射实现了导致伸长、极化和亚结构生成（类似纳米凸起结构）的光驱动变形。此外，基于高激光强度（20 - 100%，10 mW输入功率的百分比）获得了滑动和旋转变形，据此提出了基于偶氮苯聚合物的光致变形模型。此外，还证明了基于偶氮聚合物的支柱的生物相容性及其局部重塑和诱导实时细胞反应的潜力。
