Schmidt G, Owman C, Sjöberg N O, Walles B
J Auton Pharmacol. 1985 Sep;5(3):241-50. doi: 10.1111/j.1474-8673.1985.tb00125.x.
Ovaries from sexually mature virgin rabbits were dissected free on both sides and cannulated in situ via that part of the aortic segment supplying the ovarian arteries. The ovaries were mounted in a closed, recirculating perfusion system for oxygenation in vitro. Ovulation was induced by human chorionic gonadotropin (hCG) given either i.v. (100 IU) or directly into the perfusion medium (20 IU). Follicular ruptures occurred in 42 out of a total of 54 ovaries. The in vivo route of hCG administration was found to be most efficient, with ovulations occurring after a mean of 11.5 h, which corresponds well with the incidence of ovulations in situ. The prostaglandin synthesis inhibitor, indomethacin, totally abolished the hCG-induced ovulations. beta-Adrenoreceptor stimulation in vitro with terbutaline or with noradrenaline in the presence of alpha-receptor blockade with phenoxybenzamine caused a significantly increased number of gonadotropin-induced ovulations. Phentolamine or phenoxybenzamine given alone reduced the number of ruptured follicles. Noradrenaline (in the absence of hCG) and clonidine augmented the incidence of ovulation. The results indicate that local adrenoreceptor mechanisms participate in the process of ovulation, both via alpha-adrenoreceptors (probably affecting the follicular smooth musculature) and beta-adrenoreceptors (possibly through some trophic or endocrine mechanism other than a mechanical factor).
从性成熟的未交配兔子体内双侧游离出卵巢，并通过供应卵巢动脉的主动脉段部分进行原位插管。将卵巢安装在一个封闭的循环灌注系统中进行体外氧合。通过静脉注射（100国际单位）或直接注入灌注介质（20国际单位）人绒毛膜促性腺激素（hCG）诱导排卵。在总共54个卵巢中，有42个发生了卵泡破裂。发现hCG的体内给药途径最有效，平均11.5小时后发生排卵，这与原位排卵的发生率非常吻合。前列腺素合成抑制剂吲哚美辛完全消除了hCG诱导的排卵。在苯氧苄胺阻断α受体的情况下，用特布他林或去甲肾上腺素在体外刺激β肾上腺素受体，导致促性腺激素诱导的排卵数量显著增加。单独给予酚妥拉明或苯氧苄胺会减少破裂卵泡的数量。去甲肾上腺素（在无hCG的情况下）和可乐定增加了排卵的发生率。结果表明，局部肾上腺素能受体机制参与排卵过程，既通过α肾上腺素能受体（可能影响卵泡平滑肌组织），也通过β肾上腺素能受体（可能通过某种营养或内分泌机制而非机械因素）。
