Adib Yara, Serror Kevin, Pinzon Jose Amaya, Duciel Laura, Delagrange Marine, Ducos Bertrand, Boccara David, Mimoun Maurice, Chaouat Marc, Bensussan Armand, Samardzic Marina, Bagot Martine, Des Courtils Céline, Michel Laurence
Inserm UMRS_1342, Hôpital Saint-Louis, 1 Avenue Claude Vellefaux, 75010 Paris, France.
UFR de Sciences du Vivant, Université Paris Cité, 75006 Paris, France.
Cells. 2025 Jun 16;14(12):909. doi: 10.3390/cells14120909.
Macrophages participate in cutaneous wound healing by adopting M1 pro-inflammatory and M2 immunoregulatory/pro-repair phenotypes. Chronic wounds associated with a deficient macrophage response could benefit from treatments that restore an acute inflammatory response and promote healing. Calcium-alginate dressings release calcium ions, which are potent bioactivators of macrophage function in wounds. Here, the effects of two calcium-alginate dressings, Algosteril (ALG, pure Ca alginate) and Biatain Alginate (BIA, Ca alginate with carboxymethyl cellulose), and a 3 mM CaCl solution were compared in human macrophages polarized to M1 or M2. ALG and CaCl preserved monocyte viability, and BIA reduced it. Both alginates and CaCl reinforced the M1 pro-inflammatory transcriptional profile and phenotype, with significant increases in IL-6 and TNF-α secretion by ALG only. In M2 macrophages, all conditions increased the M1-specific gene expression and reduced M2 markers, suggesting an orientation toward an inflammatory profile. Only ALG significantly increased the secretion of CCL18 and VEGF, suggesting pro-repair activity. All conditions increased M2 phagocytic activity. This work demonstrates the interest in calcium alginates for stimulating macrophage subtypes, which could help restore wound healing, especially in patients with compromised innate immunity. It highlights the differences among the calcium-alginate dressings. The pure alginate shows higher stimulation of macrophage pro-inflammatory and pro-repair functions.
巨噬细胞通过呈现M1促炎和M2免疫调节/促修复表型参与皮肤伤口愈合。与巨噬细胞反应不足相关的慢性伤口可能受益于恢复急性炎症反应并促进愈合的治疗方法。海藻酸钙敷料会释放钙离子，而钙离子是伤口中巨噬细胞功能的有效生物激活剂。在此，我们比较了两种海藻酸钙敷料Algosteril（ALG，纯海藻酸钙）和Biatain藻酸盐（BIA，含羧甲基纤维素的海藻酸钙）以及3 mM氯化钙溶液对极化至M1或M2的人巨噬细胞的影响。ALG和氯化钙可维持单核细胞活力，而BIA则会降低其活力。两种海藻酸盐和氯化钙均增强了M1促炎转录谱和表型，仅ALG使IL-6和TNF-α分泌显著增加。在M2巨噬细胞中所有条件均增加了M1特异性基因表达并降低了M2标志物，表明趋向于炎症表型。只有ALG显著增加了CCL18和VEGF的分泌，表明具有促修复活性。所有条件均增加了M2吞噬活性。这项研究表明海藻酸钙在刺激巨噬细胞亚型方面具有应用价值，这可能有助于恢复伤口愈合，尤其是对于先天免疫受损患者。它突出了海藻酸钙敷料之间的差异。纯海藻酸盐对巨噬细胞促炎和促修复功能的刺激作用更强。
