Campos Aline D O, Wahalathanthrige Hashini J, Russell Shane, Harrison Mark D, Strong Peter James
Centre for Agriculture and the Bioeconomy, Queensland University of Technology, Brisbane 4000, Australia.
School of Biology and Environmental Science, Queensland University of Technology, Brisbane 4000, Australia.
J Fungi (Basel). 2025 Jun 6;11(6):432. doi: 10.3390/jof11060432.
Stillage is an acidic residue from ethanol production that has a high carbon load. Here, isolates were assessed for the treatment of rum stillage while producing biomass and associated metabolites. Isolates grew in 25% raw stillage, removing up to 73% of soluble organic carbon, 77% soluble nitrogen, and 74% phenolic compounds. Isolate G2 demonstrated faster removal of organic carbon and nitrogen. Biomass and metabolite production were benchmarked against a nutrient medium. In stillage, maximum values of the following were obtained: 8.2 g·L biomass; 52.8% crude protein; 22.1 mg·g extractable protein; antioxidants of 17.2 mg TE·g (2,2'-azino-di-(3-ethylbenzothiazoline-6-sulfonic acid), ABTS) and 16.6 µmol Fe·g (ferric reducing antioxidant power, FRAP); 2.9 mg GAE·g phenolic compounds (gallic acid equivalents); 1.2% lipids; and 11% β-glucans. In the nutrient medium, the following were obtained: 6.9 g·L biomass; 56.4% crude protein; 38.7 mg·g extractable protein; antioxidants of 24.9 mg TE·g (ABTS) and 25.9 µmol Fe·g (FRAP); 6.0 mg GAE·g phenolic compounds; 0.7% lipids; and 13% β-glucans. To our knowledge, this is the first report detailing the biomass metabolite content of mycelium using rum stillage. The production of edible biomass containing bioactive products demonstrates the potential of using strains to valorize this residue.
酒糟是乙醇生产过程中产生的一种酸性残渣，碳含量很高。在此，对分离菌株进行了评估，以确定其在处理朗姆酒酒糟的同时生产生物质及相关代谢产物的能力。分离菌株能在25%的原酒糟中生长，去除高达73%的可溶性有机碳、77%的可溶性氮和74%的酚类化合物。分离菌株G2对有机碳和氮的去除速度更快。将生物质和代谢产物的产量与营养培养基进行了对比。在酒糟中，获得了以下各项的最大值：生物量8.2 g·L；粗蛋白52.8%；可提取蛋白22.1 mg·g；抗氧化剂含量为17.2 mg TE·g（2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)，ABTS）和16.6 µmol Fe·g（铁还原抗氧化能力，FRAP）；酚类化合物2.9 mg GAE·g（没食子酸当量）；脂质1.2%；β-葡聚糖11%。在营养培养基中，获得的结果如下：生物量6.9 g·L；粗蛋白56.4%；可提取蛋白38.7 mg·g；抗氧化剂含量为24.9 mg TE·g（ABTS）和25.9 µmol Fe·g（FRAP）；酚类化合物6.0 mg GAE·g；脂质0.7%；β-葡聚糖13%。据我们所知，这是第一份详细描述利用朗姆酒酒糟生产的菌丝体中生物质代谢产物含量的报告。生产含有生物活性产品的可食用生物质证明了利用这些菌株使这种残渣增值的潜力。
