Lai C N, Becker F F
J Cell Physiol. 1985 Nov;125(2):259-62. doi: 10.1002/jcp.1041250213.
High potassium concentrations altered the morphology and the ability to grow in soft agar in 6m2 cells, a clone of rat kidney cells infected with a temperature-sensitive mutant of Moloney sarcoma virus. Approximately 60% of cells exhibited normal morphology in the presence of 94.8 mM potassium in isotonic medium at the temperature permissive for transformation, whereas 100% were normal at 72 mM potassium in hypertonic media. A significant reduction of growth in soft agar was also induced with these conditions. However, the synthesis ratio of virus-specified transforming protein to marker viral protein was not altered. Na+K+-ATPase might play a role in this reverse-transformation process.
高钾浓度改变了6m2细胞的形态以及在软琼脂中生长的能力，6m2细胞是感染了莫洛尼肉瘤病毒温度敏感突变体的大鼠肾细胞克隆。在转化允许温度下的等渗培养基中，当钾浓度为94.8 mM时，约60%的细胞呈现正常形态，而在高渗培养基中钾浓度为72 mM时，100%的细胞形态正常。这些条件还导致软琼脂中细胞生长显著减少。然而，病毒特异性转化蛋白与标记病毒蛋白的合成比率没有改变。钠钾ATP酶可能在这种逆向转化过程中起作用。
