罗马尼亚五种野生鸦科鸟类中流行的毒株及其基因型

Prevalence and Genotypes Circulating in Five Wild Corvid Species from Romania.

作者信息

Gherman Călin Mircea, Balea Anamaria, Györke Adriana, Kalmár Zsuzsa, Ionică Angela Monica, Villena Isabelle, Spano Furio, de Craeye Stéphane, Cozma Vasile

机构信息

Department of Parasitology and Parasitic Diseases, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania.

Parasitology Laboratory, Animal Health and Food Safety Agency Cluj Division, 400372 Cluj-Napoca, Romania.

出版信息

Pathogens. 2025 Jun 7;14(6):572. doi: 10.3390/pathogens14060572.

DOI:10.3390/pathogens14060572

PMID:40559580

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12196500/

Abstract

The apicomplexan parasite can potentially infect all warm-blooded animals, including birds, which, due to their high dispersal capabilities, are considered a significant candidate group of sentinel animals that reveal environmental contamination with this protozoan. In the present study, the serologic and molecular prevalences of infection were determined in 333 corvids from Romania. Paired meat juice (n = 333) and heart samples (n = 244) were collected and analyzed using the modified agglutination test for antibodies, polymerase chain reaction (PCR) for DNA, and SAG2 molecular marker sequencing for genotyping. The overall antibodies prevalence was 19.5%, with 48.1% infected jackdaws, 72.8% rooks, 89.7% hooded crows, 77.5% magpies, and 42.9% jays. Of 244 heart samples analyzed with PCR amplification, only 3 (1.2%) resulted positive and were shown to belong to genotype III through the sequencing of the SAG2 amplicon. This is the first extensive study on . in crows from Romania.

摘要

顶复门寄生虫有可能感染所有温血动物，包括鸟类，由于鸟类具有高度的扩散能力，它们被认为是揭示这种原生动物环境污染的重要哨兵动物候选群体。在本研究中，对来自罗马尼亚的333只鸦科鸟类进行了感染的血清学和分子流行率测定。采集了配对的肉汁样本（n = 333）和心脏样本（n = 244），并使用改良凝集试验检测抗体、聚合酶链反应（PCR）检测DNA以及SAG2分子标记测序进行基因分型分析。总体抗体流行率为19.5%，其中寒鸦感染率为48.1%，白嘴鸦为72.8%，秃鼻乌鸦为89.7%，喜鹊为77.5%，松鸦为42.9%。在244份经PCR扩增分析的心脏样本中，只有3份（1.2%）呈阳性，通过SAG2扩增子测序显示属于基因型III。这是罗马尼亚首次对乌鸦体内的[寄生虫名称未给出]进行的广泛研究。