Chakraborty Sourav, Baindara Piyush, Das Surojit, Mondal Suresh K, Sharma Pralay, Jose T Austin, V Kumaravel, Manoharan Raja, Mandal Santi M
Department of Bioscience and Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Animal Science Research Center, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA.
Medicines (Basel). 2025 May 22;12(2):14. doi: 10.3390/medicines12020014.
: Methicillin-resistant (MRSA) considered under the category of serious threats by the Centers for Disease Control and Prevention (CDC), urges for new antibiotics or alternate strategies to control MRSA. : Ethosome-like liposomes have been developed and characterized using dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and scanning electron microscopy (SEM). Liposomes were confirmed for antibiotics infusion by encapsulation efficiency and release kinetics as well. Further, the antimicrobial potential of liposomes was checked by determination of minimum inhibitory concentrations (MICs), crystal violet assay, and live/dead biofilm eradication assay. : The specially designed liposomes consist of amphiphilic molecules, tocopherol, conjugated with ampicillin and, another antibiotic amikacin, loaded in the core. The developed liposomes exhibited good encapsulation efficiency, and sustained release while serving as ideal antibiotic carriers for advanced efficacy along with anti-inflammatory benefits from tocopherol. Conclusively, newly designed liposomes displayed potential antimicrobial activity against MRSA and its complex biofilms. : Overall, dual antibiotic-encapsulated liposomes demonstrate the potential to eradicate MRSA and its mature biofilms by dual-targeted action. This could be developed as an efficient anti-infective agent and delivery vehicle for conventional antibiotics to combat MRSA.
耐甲氧西林金黄色葡萄球菌（MRSA）被美国疾病控制与预防中心（CDC）列为严重威胁类别，因此迫切需要新的抗生素或替代策略来控制MRSA。类乙醇脂质体已通过动态光散射（DLS）、傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）进行了开发和表征。脂质体还通过包封效率和释放动力学来确认抗生素的注入情况。此外，通过测定最低抑菌浓度（MIC）、结晶紫测定法和活菌/死菌生物膜根除测定法来检查脂质体的抗菌潜力。特别设计的脂质体由两亲性分子生育酚组成，生育酚与氨苄西林共轭，另一种抗生素阿米卡星负载在核心中。所开发的脂质体表现出良好的包封效率和持续释放，同时作为具有先进疗效的理想抗生素载体，并具有生育酚带来的抗炎益处。总之，新设计的脂质体对MRSA及其复杂生物膜显示出潜在的抗菌活性。总体而言，双重抗生素包封的脂质体通过双靶点作用显示出根除MRSA及其成熟生物膜的潜力。这可以开发成为一种有效的抗感染剂和传统抗生素的递送载体，以对抗MRSA。
