Chakraborty Sourav, Baindara Piyush, Sharma Pralay, Jose T Austin, V Kumaravel, Manoharan Raja, Mandal Santi M
Department of Bioscience and Biotechnology, Indian Institute of Technology, Kharagpur 721302, India.
Animal Sciences Research Center, Division of Animal Sciences, University of Missouri, Columbia, MO 65211, USA.
Antibiotics (Basel). 2024 Jul 24;13(8):689. doi: 10.3390/antibiotics13080689.
Hospital-acquired antibiotic-resistant pneumonia is one of the major causes of mortality around the world that pose a catastrophic threat. is one of the most significant opportunistic pathogens responsible for hospital-acquired pneumonia and gained resistance to the majority of conventional antibiotics. There is an urgent need for antibiotic alternatives to control drug-resistant pneumonia and other related respiratory infections. In the present study, we explored the antibacterial potential of cineole in combination with homeopathic medicines against biofilm-forming drug-resistant . Out of 26 selected and screened homeopathic medicines, (HyPer) was found to eradicate biofilm-forming drug-resistant most effectively when used in combination with cineole. Interestingly, the synergistic action of HyPer and cineole was also found to be similarly effective against planktonic cells of . Further, the potential synergistic killing mechanisms of cineole and HyPer were determined by analyzing zeta membrane potential, outer membrane permeability, and DNA release from cells upon treatment with cineole and HyPer. Additionally, molecular docking analysis revealed strong binding affinities of hypericin (an active ingredient of HyPer) with the PqsA (a quorum sensing protein) of . Overall, our findings revealed the potential synergistic action of cineole and HyPer against biofilm-forming drug-resistant . Cineole and HyPer could be used in combination with other bronchodilators as inhalers to control the biofilm-forming drug-resistant .
医院获得性耐抗生素肺炎是全球主要的死亡原因之一，构成了灾难性威胁。是导致医院获得性肺炎的最重要的机会性病原体之一，并且对大多数传统抗生素产生了耐药性。迫切需要抗生素替代品来控制耐药性肺炎和其他相关呼吸道感染。在本研究中，我们探索了桉叶油素与顺势疗法药物联合对形成生物膜的耐药菌的抗菌潜力。在26种经过挑选和筛选的顺势疗法药物中，发现（HyPer）与桉叶油素联合使用时，能最有效地根除形成生物膜的耐药菌。有趣的是，还发现HyPer和桉叶油素的协同作用对该菌的浮游细胞同样有效。此外，通过分析用桉叶油素和HyPer处理后该菌细胞的ζ膜电位、外膜通透性和DNA释放，确定了桉叶油素和HyPer潜在的协同杀伤机制。此外，分子对接分析显示金丝桃素（HyPer的一种活性成分）与该菌的PqsA（一种群体感应蛋白）具有很强的结合亲和力。总体而言，我们的研究结果揭示了桉叶油素和HyPer对形成生物膜的耐药菌具有潜在的协同作用。桉叶油素和HyPer可与其他支气管扩张剂联合用作吸入剂，以控制形成生物膜的耐药菌。
