Bharathi Durairaj Swarna, Raja Andiyappan Boopathy, Nachimuthu Suganthi, Thangavel S, Kannan Karthik, Shanmugan Sengottaiyan, Tari Vinaya
PG and Research Department of Zoology, Nehru Memorial College, Affiliated to Bharathidasan University, Puthanampatti 621004, Tamil Nadu, India.
PG and Research Department of Physics, Government Arts College (Autonomous), Affiliated to Bharathidasan University, Karur 639005, Tamil Nadu, India.
Mar Drugs. 2025 May 23;23(6):224. doi: 10.3390/md23060224.
This study investigates the rare seaweed alga lamour for biological activity. Antioxidant and antibacterial activities were examined. An MTT assay was carried out to examine cytotoxicity activity against colon cancer cells. The HPTLC analysis was performed for four different extracts, which exhibited clear flavonoid band formation at 254 nm and 366 nm with varied ranges of R values: methanolic extract (R 0.87), acetone extract (R 0.82), and benzene (R 0.83). Methanolic Extract Fraction One (MEF1) has a distinct band formation at 366 nm, it is shown to have the highest inhibition (6.20 ± 0.53 mm) against , and the MTT assay reveals that the aqueous extract of extract has an IC value of 300 µg/mL. It is divulged that methanolic extract shows the highest phytochemical compound level among the four extracts of . A GC/MS analysis was employed to investigate the flavonoid profile of the crude seaweed extract. Although LC/MS is typically preferred for flavonoid analysis due to thermal sensitivity, GC/MS was used in this study owing to time constraints, with optimized conditions to reduce thermal degradation. The GC-MS analysis identified Quinoline and other flavonoids, suggesting potential bioactivity. The cytotoxicity activity of MEF1 shows that the development of a promising drug may be evaluated from a seaweed source. The present study provides excellent insight with the first report of the biologically active compound of .
本研究调查了珍稀海藻拉穆尔藻的生物活性。检测了其抗氧化和抗菌活性。进行了MTT试验以检测对结肠癌细胞的细胞毒性活性。对四种不同提取物进行了高效薄层色谱分析，这些提取物在254nm和366nm处呈现出清晰的黄酮带形成，R值范围各异：甲醇提取物（R 0.87）、丙酮提取物（R 0.82）和苯提取物（R 0.83）。甲醇提取物组分一（MEF1）在366nm处有明显的条带形成，显示对[具体对象未明确]具有最高抑制作用（6.20±0.53mm），MTT试验表明该提取物的水提取物IC值为300μg/mL。结果表明，甲醇提取物在所研究的四种提取物中显示出最高的植物化学化合物水平。采用气相色谱/质谱联用（GC/MS）分析来研究粗海藻提取物的黄酮类成分。尽管由于热敏感性，液相色谱/质谱联用（LC/MS）通常更适合黄酮类分析，但由于时间限制，本研究使用了GC/MS，并采用了优化条件以减少热降解。GC-MS分析鉴定出喹啉和其他黄酮类化合物，表明其具有潜在的生物活性。MEF1的细胞毒性活性表明，有望从海藻来源开发出一种有前景的药物。本研究首次报道了[具体海藻名称未明确]的生物活性化合物，提供了极好的见解。
