Guevara-Torrejón Valeska, Chandía Parra Paola, Campos-Estrada Carolina, Vera Quezada Waleska E
Laboratorio de Química de Metabolitos Bioactivos, Facultad de Farmacia, Universidad de Valparaíso, Valparaíso 2340000, Chile.
Laboratorio de Moléculas Bioactivas, Facultad de Ciencias del Mar, Universidad Católica del Norte, Coquimbo 1780000, Chile.
Mar Drugs. 2025 Jun 11;23(6):248. doi: 10.3390/md23060248.
The ulvans are sulfated heteropolysaccharides that can stimulate the immune response in vitro. Using a human cell model, this study aimed to characterize and evaluate the immunostimulatory properties of crude ulvans extracted from spp., collected in Algarrobo, Chile. The crude ulvans, characterized by spectrophotometric methods, are composed of 47.6% total sugars, 14.3% uronic acids, and 8.9% sulfates, with an average molecular weight of 40.000 kDa. The FTIR spectrum showed bands related to uronic acids, rhamnose, and sulfate groups. GCMS analysis confirmed the presence of rhamnose, xylose, glucose, and galactose, with a predominance of the disaccharides U3s and B3s. HL60 cells differentiated into macrophages were cultured with three concentrations of crude ulvans (25, 50, and 100 μg/mL), with cell viability remaining above 90% at the lower concentrations. The crude ulvan activated CD86 co-stimulatory molecules and promoted the release of IL-6, IL-10, IL-4, and nitric oxide cytokines. The results suggest that ulvan is non-toxic and can activate inflammatory pathways, making it a potential candidate for studies as a vaccine adjuvant.
石莼多糖是硫酸化杂多糖，可在体外刺激免疫反应。本研究使用人类细胞模型，旨在表征和评估从智利阿尔加罗沃采集的石莼属物种中提取的粗石莼多糖的免疫刺激特性。通过分光光度法表征的粗石莼多糖由47.6%的总糖、14.3%的糖醛酸和8.9%的硫酸盐组成，平均分子量为40.000 kDa。傅里叶变换红外光谱显示了与糖醛酸、鼠李糖和硫酸根基团相关的谱带。气相色谱-质谱分析证实了鼠李糖、木糖、葡萄糖和半乳糖的存在，其中二糖U3s和B3s占主导。将分化为巨噬细胞的HL60细胞与三种浓度的粗石莼多糖（25、50和100μg/mL）一起培养，较低浓度下细胞活力保持在90%以上。粗石莼多糖激活了CD86共刺激分子，并促进了IL-6、IL-10、IL-4和一氧化氮细胞因子的释放。结果表明，石莼多糖无毒，可激活炎症途径，使其成为作为疫苗佐剂研究的潜在候选物。
