Thomas Angelos-Lauris, Karayannopoulou Maria, Anagnostou Tilemachos, Psalla Dimitra, Ioannou Konstantinos, Ginoudis Argyrios, Savvas Ioannis, Pardali Dimitra
Department of Clinical Studies-Companion Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 11 St. Voutyra Street, 546 27 Thessaloniki, Greece.
Laboratory of Pathology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, University Campus, 541 24 Thessaloniki, Greece.
Vet Sci. 2025 Jun 8;12(6):562. doi: 10.3390/vetsci12060562.
In cancer cases, any imbalance between Reactive Oxygen Species (ROS) production and host antioxidant capacity (oxidative stress), during the immediate post-operative period, is involved in cancer cell dissemination (micro-metastases). Surgical trauma is considered the main cause for ROS production during that period. Increased oxidative stress has been found in dogs with mammary neoplasms. Our objective was to investigate the blood oxidant/antioxidant profile in bitches with mammary cancer, during the peri-operative period, hypothesizing that oxidative stress would be further increased after mastectomy. Twelve bitches with mammary cancer and no distant metastases, with a comparable extent of surgical wound and receiving the same anesthetic protocol, were included. Their serum oxidant/antioxidant profile was evaluated chromatometrically using the d-ROMs (Reactive Oxygen Metabolite-derived compounds) and the BAP (biological antioxidant potential) tests, on the 1st day (before/after surgery), and on the 2nd, 3rd and 10th days post-operatively. The ratio of d-ROMs and BAP values (oxidative stress index; OSI) was also calculated. Statistically significant differences (increases) were found only in d-ROMS on the second and third post-operative day compared to the first day (before surgery) of measurement ( = 0.007 and = 0.002, respectively). Our findings indicate a post-surgical overproduction of ROS and therefore an oxidative stress increase potentially affecting patient outcome.
在癌症病例中，术后即刻活性氧（ROS）生成与宿主抗氧化能力（氧化应激）之间的任何失衡都与癌细胞扩散（微转移）有关。手术创伤被认为是该时期ROS生成的主要原因。在患有乳腺肿瘤的犬中已发现氧化应激增加。我们的目的是研究患有乳腺癌的母犬围手术期的血液氧化/抗氧化谱，假设乳房切除术后氧化应激会进一步增加。纳入了12只无远处转移、手术伤口范围相当且接受相同麻醉方案的患有乳腺癌的母犬。在第1天（手术前/后）以及术后第2、3和10天，使用d-ROMs（活性氧代谢产物衍生化合物）和BAP（生物抗氧化潜力）测试通过色谱法评估它们的血清氧化/抗氧化谱。还计算了d-ROMs与BAP值的比率（氧化应激指数；OSI）。与测量的第一天（手术前）相比，仅在术后第二天和第三天的d-ROMS中发现了统计学上的显著差异（增加）（分别为 = 0.007和 = 0.002）。我们的研究结果表明术后ROS产生过量，因此氧化应激增加可能会影响患者的预后。
