Chang Penghuan, Huang Hui, Huang Peijian, Yi Hongru, Zhong Wenhua, Yang Zhaoxin
Department of Nursing, Haikou Affiliated Hospital of Central South University, Xiangya School of Medicine, Haikou, China.
College of Pharmacy, Hainan Medical University, Haikou, China.
Braz J Med Biol Res. 2025 Jun 20;58:e14651. doi: 10.1590/1414-431X2025e14651. eCollection 2025.
Total hysterectomy is a commonly performed gynecological procedure used to treat uterine pathologies. This study aimed to establish a total hysterectomy mouse model and investigate the effect of estradiol valerate on humoral immune function in BALB/c mice. Our results showed that the ratios of CD4+ and CD8+ subpopulation and hepatitis B virus antibody IgG content were reduced in the total hysterectomy (TH) group compared with the control group (P<0.05). The measured indices showed varying degrees of improvement after estradiol valerate administration. The numbers of CD19+ B lymphocytes significantly increased in the TH group (P<0.05) compared with the control group. There was a significant reduction of the size of germinal centers within lymph nodes in the TH group. This effect was reversed by estrogen supplementation. In summary, reduced estrogen levels following total hysterectomy may impair B lymphocyte activation, IgG secretion, and plasma cell production, leading to compromised immune function. Early treatment with estradiol valerate reverses impaired immune function and enhances antigen-induced antibody production in mice.
全子宫切除术是一种常用于治疗子宫疾病的妇科手术。本研究旨在建立全子宫切除小鼠模型，并研究戊酸雌二醇对BALB/c小鼠体液免疫功能的影响。我们的结果表明，与对照组相比，全子宫切除（TH）组的CD4+和CD8+亚群比例以及乙肝病毒抗体IgG含量降低（P<0.05）。给予戊酸雌二醇后，所测指标显示出不同程度的改善。与对照组相比，TH组CD19+B淋巴细胞数量显著增加（P<0.05）。TH组淋巴结内生发中心大小显著减小。补充雌激素可逆转这种作用。总之，全子宫切除术后雌激素水平降低可能会损害B淋巴细胞活化、IgG分泌和浆细胞产生，导致免疫功能受损。早期用戊酸雌二醇治疗可逆转受损的免疫功能，并增强小鼠抗原诱导的抗体产生。
