富血小板纤维蛋白补充甲硝唑对具核梭杆菌的体外抗菌效果。
The in vitro antibacterial efficacy of advanced platelet-rich fibrin plus supplemented with metronidazole against Fusobacterium nucleatum.
作者信息
Huynh-Pham Anh-Truc, Le Son Hoang, Nguyen Bich-Ly Thi
机构信息
Department of Oral Surgery, Faculty of Dentistry, University of Medicine and Pharmacy at Ho Chi Minh City, 652 Nguyen Trai Street, Cho Lon Ward, Ho Chi Minh City, 72714, Vietnam.
出版信息
Clin Oral Investig. 2025 Jun 26;29(7):359. doi: 10.1007/s00784-025-06431-0.
PURPOSE
Owing to its high antibacterial efficacy and low adverse effects, local antibiotic administration has attracted attention. This in vitro study compares the antibacterial effectiveness of advanced platelet-rich fibrin plus (A-PRF+) combined with metronidazole produced under different protocols.
MATERIALS AND METHODS
Eight volunteers participated in this study. A 0.02% metronidazole was supplemented into A-PRF + based on four protocols concerning time (before vs. after centrifugation) and dosages (0.25 vs. 0.50 mL), yielding four groups: B25 (before centrifugation, 0.25 mL), B50 (before centrifugation, 0.50 mL), A25 (after centrifugation, 0.25 mL), and A50 (after centrifugation, 0.50 mL). Agar diffusion experiments on Fusobacterium nucleatum strain ATCC 25,586 were conducted to determine the bacterial efficacy of the four groups on three time points: day 2 (D2), D3 and D4. Images of the bacterial inhibition zone (BIZ) at assessment times were captured to measure BIZ dimensions using ImageJ software.
RESULTS
No significant difference was found between the BIZs of B25 and B50; however, the BIZs of A50 were significantly higher than those of A25. BIZs of each group tend to decrease, although no significant difference was found. Two-way repeated measures ANOVA revealed a significant interaction between the timing and observation time points. Post-hoc analysis showed that at each observation time point, BIZs of A-PRF + combined with metronidazole after centrifugation were significantly higher than those before, regardless of the dosage.
CONCLUSION
The results of this study suggest that supplementing metronidazole into A-PRF + after centrifugation provides better antibacterial efficacy against Fusobacterium nucleatum than before centrifugation.
CLINICAL RELEVANCE
A-PRF + supplemented with metronidazole after centrifugation can exert better antibacterial efficacy against Fusobacterium nucleatum.
目的
由于局部应用抗生素具有高抗菌疗效和低不良反应，已受到关注。本体外研究比较了在不同方案下制备的富血小板纤维蛋白升级版（A-PRF+）联合甲硝唑的抗菌效果。
材料与方法
8名志愿者参与本研究。基于时间（离心前与离心后）和剂量（0.25 vs. 0.50 mL）的四个方案，将0.02%的甲硝唑添加到A-PRF+中，产生四组：B25（离心前，0.25 mL）、B50（离心前，0.50 mL）、A25（离心后，0.25 mL）和A50（离心后，0.50 mL）。对具核梭杆菌ATCC 25586菌株进行琼脂扩散实验，以确定这四组在三个时间点（第2天、第3天和第4天）的抗菌效果。在评估时间拍摄细菌抑制圈（BIZ）的图像，使用ImageJ软件测量BIZ尺寸。
结果
B25和B50的BIZ之间未发现显著差异；然而，A50的BIZ显著高于A25。每组的BIZ均有下降趋势，尽管未发现显著差异。双向重复测量方差分析显示时间和观察时间点之间存在显著交互作用。事后分析表明，在每个观察时间点，无论剂量如何，离心后A-PRF+联合甲硝唑的BIZ均显著高于离心前。
结论
本研究结果表明，离心后在A-PRF+中添加甲硝唑对具核梭杆菌的抗菌效果优于离心前。
临床意义
离心后添加甲硝唑的A-PRF+对具核梭杆菌可发挥更好的抗菌效果。
Zotero 插件