Báez-Ruiz Adrián, Hernández-Abrego Andy, Díaz-Muñoz Mauricio, Méndez Isabel
Laboratorio de Ritmos Biológicos, Facultad de Ciencias, Universidad Autónoma de San Luis Potosí, Avenida Chapultepec 1570, Privadas del Pedregal, San Luis Potosí 78295, Mexico.
Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología, Universidad Nacional Autónoma de México (UNAM), Campus UNAM-Juriquilla, Boulevard Juriquilla 3001, Querétaro 76230, Mexico.
Antioxidants (Basel). 2025 May 28;14(6):649. doi: 10.3390/antiox14060649.
Daytime-restricted feeding (TRF) exerts outstanding effects on circadian physiology, nutrient utilization, and energy metabolism. Limiting feeding access to two hours during the daytime (12:00-14:00 h) for three weeks promotes food-anticipatory activity (FAA). FAA encompasses not only behaviors related to meal expectations but also includes diurnal fluctuations in liver metabolic responses, including distinct redox handling. Hepatic microarray profiles of genes associated with redox response processes were analyzed at three crucial time points: at the beginning of the light period or before FAA (08:00 h), during the expression of FAA (11:00 h), and after feeding (14:00 h). Data on fasting and nutrient processing were integrated, whereas circadian implications were extrapolated by comparing the TRF transcriptional output with a one-day fasting group. Transcripts of redox reactions, such as reactive oxygen species (ROS) generation, antioxidant defenses, NAD/NADH equilibrium, and glutathione, hydrogen peroxide (HO), arginine, nitric oxide (NO), and hydrogen sulfide (HS) metabolism, were analyzed. Results showed a decline in antioxidant defenses at 08:00 h, followed by a burst of pro-oxidant reactions, preparation of glutathione metabolism factors, and a tendency to decrease HO and increase NO and HS during the FAA. Most of the findings observed during the FAA were absent in response to one-day fasting. Hence, TRF involves concerted and sequential responses in liver pro-oxidant and antioxidant reactions, facilitating a redox-related circadian control that optimizes the metabolic utilization of nutrients, which differs from a response to a simple fast-feed cycle.
限时进食（TRF）对昼夜节律生理、营养利用和能量代谢具有显著影响。将进食时间限制在白天的两个小时（12:00 - 14:00），持续三周，可促进食物预期活动（FAA）。FAA不仅包括与进食预期相关的行为，还包括肝脏代谢反应的昼夜波动，包括独特的氧化还原处理。在三个关键时间点分析了与氧化还原反应过程相关基因的肝脏微阵列图谱：光照期开始时或FAA之前（08:00）、FAA表达期间（11:00）以及进食后（14:00）。整合了禁食和营养处理的数据，同时通过将TRF转录输出与一日禁食组进行比较来推断昼夜节律的影响。分析了氧化还原反应的转录本，如活性氧（ROS）生成、抗氧化防御、NAD/NADH平衡以及谷胱甘肽、过氧化氢（HO）、精氨酸、一氧化氮（NO）和硫化氢（HS）代谢。结果显示，08:00时抗氧化防御能力下降，随后促氧化反应激增，谷胱甘肽代谢因子开始准备，并且在FAA期间HO有下降趋势，NO和HS有上升趋势。在FAA期间观察到的大多数结果在一日禁食的反应中并未出现。因此，TRF涉及肝脏促氧化和抗氧化反应的协同和顺序反应，促进了与氧化还原相关的昼夜节律控制，从而优化了营养物质的代谢利用，这与对简单禁食 - 进食周期的反应不同。
