Pu Aoyang, Sim Woo-Sup, Liem Yuen-Kei, Lai Yimin, Park Bong-Woo, Lee Kyoung-Tae, Park Hun-Jun, Ban Kiwon
Department of Biomedical Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
Tung Biomedical Sciences Centre, City University of Hong Kong, Hong Kong SAR, China.
Antioxidants (Basel). 2025 May 31;14(6):671. doi: 10.3390/antiox14060671.
(GA), a polyphenolic compound derived from amur maple trees, has been identified as a powerful scavenger of reactive oxygen species (ROS). Recognizing the pivotal role of ROS in exacerbating secondary damage during myocardial ischemia-reperfusion injury (MIRI), we fractionated GA-enriched extracts from the leaves of the amur maple tree, L. subsp. () , using common solvents of dichloromethane (DCM) and ethyl acetate (EA). When co-administered for 30 min, the DCM- and EA-fractioned extracts effectively protected cardiomyocytes from HO-induced damage. ROS-sensitive probes indicated that treatment with extracts significantly reduced both intracellular and mitochondrial ROS levels. Instead of enhancing the activity of antioxidative enzymes, the extracts acted as natural antioxidases, directly scavenging various ROS such as superoxide, HO, hydroxyl radical, and Fe within just 20 min. In a MIRI rat model, the in vivo administration of extracts provided significant cardioprotection by preserving viable myocardia and enhancing cardiac functions. Additionally, treatment with extracts significantly reduced cardiac fibrosis and denatured collagen. Our study suggests that the ultrafast ROS scavenging capability of extracts offers substantial heart protection during MIRI. Incorporating extracts as a pharmacological intervention during reperfusion could effectively mitigate ROS-induced cardiac injury.
源自茶条槭树的多酚类化合物没食子酸（GA）已被确定为活性氧（ROS）的强力清除剂。鉴于ROS在心肌缺血再灌注损伤（MIRI）期间加剧继发性损伤中起关键作用，我们使用二氯甲烷（DCM）和乙酸乙酯（EA）等常用溶剂，从茶条槭亚种（L. subsp. ()）的叶子中分离出富含GA的提取物。当共同给药30分钟时，DCM和EA分离的提取物有效保护心肌细胞免受HO诱导的损伤。ROS敏感探针表明，提取物处理显著降低了细胞内和线粒体ROS水平。提取物并非增强抗氧化酶的活性，而是充当天然抗氧化剂，在短短20分钟内直接清除各种ROS，如超氧化物、HO、羟基自由基和铁。在MIRI大鼠模型中，提取物的体内给药通过保留存活心肌和增强心脏功能提供了显著的心脏保护作用。此外，提取物处理显著减少了心脏纤维化和变性胶原蛋白。我们的研究表明，提取物的超快ROS清除能力在MIRI期间提供了实质性的心脏保护。在再灌注期间将提取物作为一种药理干预措施纳入，可有效减轻ROS诱导的心脏损伤。
