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ZC529通过激活Keap1-Nrf2信号通路和抑制NF-κB信号通路来保护肠道上皮屏障完整性。

ZC529 Protects Intestinal Epithelial Barrier Integrity by Activating the Keap1-Nrf2 Signaling Pathway and Inhibiting the NF-κB Signaling Pathway.

作者信息

Yuan Zian, Huang Lang, Hu Zhenguo, Deng Junhao, Duan Yehui, Jiang Qian, Tan Bi'e, Ma Xiaokang, Zhang Chen, Tang Xiongzhuo

机构信息

Hunan Provincial Key Laboratory for the Products Quality Regulation of Livestock and Poultry, College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.

Yuelushan Laboratory, Changsha 410128, China.

出版信息

Antioxidants (Basel). 2025 Jun 14;14(6):732. doi: 10.3390/antiox14060732.

DOI:10.3390/antiox14060732
PMID:40563364
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12189399/
Abstract

The probiotic bacteria ZC529 ( ZC529) has been identified from the colon of the Diannan small-ear (DSE) pig, but its intestinal protective function still lacks investigation. Here, we established a dextran sodium sulfate (DSS)-induced intestinal oxidative stress model in both and porcine small intestinal epithelial (IPEC-J2) cell lines to explore the anti-oxidative and anti-inflammatory effects of ZC529. The data showed that the intestinal colonization of ZC529 counteracted DSS-induced intestinal oxidative stress and excessive reactive oxygen species (ROS) generation by activation of the CncC pathway, a homology of the nuclear factor erythroid 2-related factor 2 (Nrf2) in mammalian systems. Moreover, ZC529 supplementation prevented flies from DSS-induced intestinal barrier damage, inflammation, abnormal excretory function, and shortened lifespan. Finally, ZC529 also attenuated DSS-induced intestinal injury in the IPEC-J2 cell line by activating the Keap1-Nrf2 signaling and inhibiting the NF-κB signaling pathways. Together, this study unraveled the profound intestinal protective function of ZC529 and provides its potential application as a new antioxidant in improving animal intestinal health as well as in developing a new probiotic in the food industry.

摘要

益生菌菌株ZC529已从滇南小耳猪的结肠中分离得到，但其肠道保护功能仍有待研究。在此，我们在果蝇和猪小肠上皮（IPEC-J2）细胞系中建立了葡聚糖硫酸钠（DSS）诱导的肠道氧化应激模型，以探究ZC529的抗氧化和抗炎作用。数据表明，ZC529在肠道的定殖通过激活CncC途径（哺乳动物系统中核因子红细胞2相关因子2（Nrf2）的同源物）来对抗DSS诱导的肠道氧化应激和过量活性氧（ROS）的产生。此外，补充ZC529可防止果蝇出现DSS诱导的肠道屏障损伤、炎症、排泄功能异常和寿命缩短。最后，ZC529还通过激活Keap1-Nrf2信号并抑制NF-κB信号通路减轻了DSS诱导的IPEC-J2细胞系中的肠道损伤。总之，本研究揭示了ZC529具有深远的肠道保护功能，并为其作为一种新型抗氧化剂改善动物肠道健康以及在食品工业中开发新型益生菌提供了潜在应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/6bb7bea5edc2/antioxidants-14-00732-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/7d68b83cf74b/antioxidants-14-00732-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/3ac06f6f1cdf/antioxidants-14-00732-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/8de9a5ac2ec1/antioxidants-14-00732-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/872fafc4fbc7/antioxidants-14-00732-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/606d8bc36b2a/antioxidants-14-00732-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/6bb7bea5edc2/antioxidants-14-00732-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/7d68b83cf74b/antioxidants-14-00732-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/3ac06f6f1cdf/antioxidants-14-00732-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/8de9a5ac2ec1/antioxidants-14-00732-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/872fafc4fbc7/antioxidants-14-00732-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/606d8bc36b2a/antioxidants-14-00732-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/738a/12189399/6bb7bea5edc2/antioxidants-14-00732-g006.jpg

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本文引用的文献

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Antioxidants (Basel). 2024 Jun 27;13(7):783. doi: 10.3390/antiox13070783.
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N4BP1 coordinates ubiquitin-dependent crosstalk within the IκB kinase family to limit Toll-like receptor signaling and inflammation.N4BP1 协调 IκB 激酶家族内的泛素依赖性串扰，以限制 Toll 样受体信号转导和炎症。
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Orally Administered CY12 Alleviates DSS-Induced Colitis in Mice by Restoring the Intestinal Barrier and Inhibiting the TLR4-MyD88-NF-κB Pathway via Intestinal Microbiota Modulation.
口服CY12通过调节肠道微生物群恢复肠道屏障并抑制TLR4-MyD88-NF-κB途径，从而减轻小鼠的右旋糖酐硫酸钠诱导的结肠炎。
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