Jin Yuxin, Niu Zehui, Feng Menglin, Che Huilian, Liang Zhihong
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Sichuan Chengdu Advanced Agricultural & Industrial Institute, China Agricultural University, Chengdu 611430, China.
Antioxidants (Basel). 2025 Jun 16;14(6):737. doi: 10.3390/antiox14060737.
() has attracted widespread attention due to its antimicrobial and anti-inflammatory properties. In this study, we prepared postbiotics from the heat-inactivated cells (HIC) and cell-free supernatant (CFS) of , with the important component L-arginine (Arg) from the metabolic products included as one of the experimental groups. The results showed that in LPS-stimulated Caco-2 cells, HIC, CFS, and Arg protect intestinal epithelial barrier integrity by inhibiting the expression of TNF-α, IL-1β, and IL-6 while enhancing the expression of occludin and ZO-1 proteins. In dextran sulfate sodium (DSS)-induced colitis mice, HIC, CFS, and Arg alleviate symptoms such as weight loss and colonic damage while suppressing the upregulation of pro-inflammatory factors and the downregulation of tight junction proteins. Moreover, these postbiotics help restore the gut microbiota composition and functionality in colitis mice, with potentially superior regulatory effects compared to sulfasalazine (SASP). Overall, HIC and CFS protect the intestinal barrier function and improve DSS-induced colitis, supporting the development of functional food supplements.
（）因其抗菌和抗炎特性而受到广泛关注。在本研究中，我们从（）的热灭活细胞（HIC）和无细胞上清液（CFS）中制备后生元，并将代谢产物中的重要成分L-精氨酸（Arg）作为实验组之一。结果表明，在LPS刺激的Caco-2细胞中，HIC、CFS和Arg通过抑制TNF-α、IL-1β和IL-6的表达，同时增强闭合蛋白和ZO-1蛋白的表达来保护肠上皮屏障完整性。在葡聚糖硫酸钠（DSS）诱导的结肠炎小鼠中，HIC、CFS和Arg减轻体重减轻和结肠损伤等症状，同时抑制促炎因子的上调和紧密连接蛋白的下调。此外，这些后生元有助于恢复结肠炎小鼠的肠道微生物群组成和功能，与柳氮磺胺吡啶（SASP）相比可能具有更好的调节作用。总体而言，HIC和CFS保护肠道屏障功能并改善DSS诱导的结肠炎，支持功能性食品补充剂的开发。
