Holmdahl R, Moran T, Andersson M
J Immunol Methods. 1985 Nov 7;83(2):379-84. doi: 10.1016/0022-1759(85)90260-1.
A simple, time-saving and efficient immunization method suitable for the production of mouse monoclonal antibody secreting hybridomas is described. Draining lymph nodes isolated 9 days after a primary immunization were used as the source of antibody producing cells. No systemic spread of antibody producing cells or specific antibodies could be detected. The present protocol was employed to generate a panel of collagen type II reactive monoclonal antibodies. Most of the monoclonals so generated were found to be of the IgG class.
描述了一种适用于生产分泌小鼠单克隆抗体的杂交瘤的简单、省时且高效的免疫方法。初次免疫9天后分离的引流淋巴结用作抗体产生细胞的来源。未检测到抗体产生细胞或特异性抗体的全身扩散。采用本方案生成了一组抗II型胶原反应性单克隆抗体。发现如此产生的大多数单克隆抗体属于IgG类。
