Holmdahl R, Andersson M, Tarkowski A
Immunology. 1987 Jul;61(3):369-74.
DBA/1 mice develop systemic polyarthritis and high titres of collagen autoantibodies after immunization with type II collagen. In this study we have analysed the frequency and specificity of antibody-producing B cells in draining lymph nodes 11 days after a primary immunization with rat type II collagen. A remarkably high frequency of type II collagen-reactive hybridomas (30%, n = 71), the majority cross-reacting with autologous type II collagen, were generated. Most of the collagen-reactive hybridomas produced IgG antibodies specific for type II collagen. Many other hybridomas produced multispecific antibodies (9%): these were preferentially of the IgM class. Some of the monoclonal multispecific hybridomas produced antibodies that cross-reacted with specific epitopes on the collagen molecule. One type II collagen-reactive hybridoma also displayed rheumatoid factor activity. Different mechanisms for the generation of the high frequency of collagen reactive antibodies are discussed.
用II型胶原蛋白免疫后，DBA/1小鼠会发生全身性多关节炎并产生高滴度的胶原蛋白自身抗体。在本研究中，我们分析了用大鼠II型胶原蛋白初次免疫11天后引流淋巴结中产生抗体的B细胞的频率和特异性。产生了频率极高的II型胶原蛋白反应性杂交瘤（30%，n = 71），其中大多数与自体II型胶原蛋白发生交叉反应。大多数胶原蛋白反应性杂交瘤产生针对II型胶原蛋白的IgG抗体。许多其他杂交瘤产生多特异性抗体（9%）：这些抗体优先为IgM类。一些单克隆多特异性杂交瘤产生的抗体与胶原蛋白分子上的特定表位发生交叉反应。一个II型胶原蛋白反应性杂交瘤还表现出类风湿因子活性。文中讨论了产生高频率胶原蛋白反应性抗体的不同机制。
