Llorens-Berzosa Sergio, Baz-González Edgar, Martin-Carrillo Natalia, García-Livia Katherine, Amaro-Ramos Virginia, Abreu-Acosta Néstor, Del Aguila Carmen, Miquel Jordi, Pino-Vera Román, Abreu-Yanes Estefanía, Feliu Carlos, Izquierdo Fernando, Foronda Pilar
Facultad de Farmacia, Universidad San Pablo-CEU, CEU Universities, Urbanización Montepríncipe, Boadilla del Monte, 28660 Madrid, Spain.
Departamento de Obstetricia y Ginecología, Pediatría, Medicina Preventiva y Salud Pública, Toxicología, Medicina Legal y Forense y Parasitología, Universidad de La Laguna (ULL), Avda. Astrofísico F. Sánchez, s/n, 38203 San Cristóbal de La Laguna, Spain.
Animals (Basel). 2025 Jun 8;15(12):1695. doi: 10.3390/ani15121695.
Rodents are recognized as reservoirs of a wide range of pathogens, including microsporidia. The presence of microsporidia in the environment of mainland Spain and its islands has become increasingly known, as the number of studies has multiplied over time. The present study was conducted to determine the occurrence and diversity of microsporidia in three rodent species (, , and ) in the Canary Islands, Spain. Ninety-three fecal samples were obtained from wild rodents on La Gomera and Gran Canaria Islands. Each sample was tested using Weber's modified trichrome staining and immunofluorescence antibody tests (IFATs) against the genus and . The microscopy-positive samples were subsequently analyzed using a nested polymerase chain reaction (PCR) followed by Sanger sequencing. The staining technique showed 38.7% (36/93) positivity, whereas the IFATs for spp. and revealed 3.2% (3/93) and 6.5% (6/93) positivity, respectively. Finally, the nested PCR and nucleotide sequence analysis confirmed a 9.7% (9/93) occurrence of and 17.2% occurrence (16/93) of different undetermined microsporidia species, whereas no spp. were detected. Seven different genotypes were detected as follows: three known (AAE1, D, and SBM1) and four novel (GRE1, GRE2, LGE1, and LGE2), all of which belonged to Group 1. The results demonstrate, for the first time, that microsporidia are present in the rodent populations of the Canary Islands. Further studies are needed to determine the impact of the presence of microsporidia in rodents on the zoonotic transmission of these parasites.
啮齿动物被认为是包括微孢子虫在内的多种病原体的宿主。随着时间的推移，研究数量不断增加，西班牙大陆及其岛屿环境中微孢子虫的存在已越来越为人所知。本研究旨在确定西班牙加那利群岛三种啮齿动物物种（、和）中微孢子虫的发生情况和多样性。从戈梅拉岛和大加那利岛的野生啮齿动物身上采集了93份粪便样本。每个样本都使用韦伯改良三色染色法和针对属和的免疫荧光抗体试验（IFAT）进行检测。随后，对显微镜检查呈阳性的样本进行巢式聚合酶链反应（PCR），然后进行桑格测序分析。染色技术显示阳性率为38.7%（36/93），而针对 spp. 和的IFAT分别显示阳性率为3.2%（3/93）和6.5%（6/93）。最后，巢式PCR和核苷酸序列分析证实，的发生率为9.7%（9/93），不同的未确定微孢子虫物种的发生率为17.2%（16/93），而未检测到 spp.。检测到七种不同的基因型，如下所示：三种已知基因型（AAE1、D和SBM1）和四种新基因型（GRE1、GRE2、LGE1和LGE2），所有这些基因型都属于第1组。结果首次证明，加那利群岛的啮齿动物种群中存在微孢子虫。需要进一步研究以确定啮齿动物中微孢子虫的存在对这些寄生虫人畜共患传播的影响。
