Wegner Marcin, Żurek Adrian, Frischke-Krajewska Joanna, Gesek Michał
Boehringer-Ingelheim, 00-728 Warsaw, Poland.
Vetdiagnostica Sp. z o.o., Otorowo 30, 86-050 Solec Kujawski, Poland.
Animals (Basel). 2025 Jun 11;15(12):1722. doi: 10.3390/ani15121722.
Infectious bursal disease (IBD) is a viral disease that most commonly affects young chickens and destroys lymphocytes, leading to immunosuppression. The field study aimed to investigate the effect of three different vaccines administered in ovo against IBD and spray against Newcastle disease (ND) on serological response tested for IBD and ND and histopathological analysis of the bursa of Fabricius (BF) and quantitative B lymphocytes in BF in broiler chickens. The study was conducted on a farm of four hen houses with 30,000 chicks in each building. Three different vaccination programs were used in the poultry hatchery, and one hen house IV was not vaccinated. All three groups were vaccinated at 18 days and 9 h in ovo during egg transfer against IBD at a dose of 0.05 mL/embryo, group I vector vaccine (strain vHVT013-69), group II immunocomplex vaccine (strain Winterfield 2512), group III immunocomplex vaccine (strain M.B, 0.05). Then, after hatching, the chicks were vaccinated in a spray (groups I, II, and III) against NDV (strain VG/GA, 20 mL/100 birds) and infectious bronchitis (IBV) in a spray (strain H-120, serotype Mass, and strain CR88121, serotype 793B) at a dose of 20 mL/100 chicks. On days 1, 21, 31, and 41, blood was collected for serological tests to determine the antibody titer against IBD, which was performed using two tests (IDEXX and ID-Vet) and against ND. During the necropsy of birds on days 21 and 31, the bursae of Fabricius were collected from five chickens for histopathological evaluation of BF and quantitative B lymphocyte counts; a total of 40 bursae were analyzed (10 per group). The vaccination program applied significantly ( < 0.05) affected the immune response expressed as a geometric mean titer (GMT) in the serum of the examined chickens against IBDV on days 21, 31, and 41. Differences were also demonstrated in the mass and level of BF damage and the number of B lymphocytes. No significant differences were demonstrated in the GMT in the serum of the examined chickens against NDV depending on the vaccination program applied.
传染性法氏囊病（IBD）是一种病毒性疾病，最常影响幼鸡并破坏淋巴细胞，导致免疫抑制。该田间研究旨在调查三种不同的卵内接种抗IBD疫苗和喷雾接种抗新城疫（ND）疫苗对肉鸡IBD和ND血清学反应、法氏囊（BF）组织病理学分析以及BF中B淋巴细胞定量的影响。该研究在一个有四个鸡舍的农场进行，每个鸡舍有30000只雏鸡。家禽孵化场采用了三种不同的疫苗接种方案，其中鸡舍IV未接种疫苗。所有三组在鸡蛋转移过程中的18日龄9小时进行卵内接种抗IBD疫苗，剂量为0.05 mL/胚胎，第一组为载体疫苗（vHVT013 - 69株），第二组为免疫复合物疫苗（Winterfield 2512株），第三组为免疫复合物疫苗（M.B株，0.05）。然后，雏鸡孵化后，第一组、第二组和第三组进行喷雾接种抗新城疫病毒（NDV，VG/GA株，20 mL/100只鸡）和喷雾接种传染性支气管炎病毒（IBV，H - 120株，Mass血清型，CR88121株，793B血清型），剂量为20 mL/100只雏鸡。在第1、21、31和41天采集血液进行血清学检测，以确定抗IBD的抗体滴度（使用IDEXX和ID - Vet两种检测方法）以及抗ND的抗体滴度。在第21天和31天对鸡进行剖检时，从五只鸡采集法氏囊进行BF组织病理学评估和B淋巴细胞定量计数；共分析了40个法氏囊（每组10个）。所应用的疫苗接种方案对所检测鸡血清中抗传染性法氏囊病病毒（IBDV）的几何平均滴度（GMT）所表示的免疫反应有显著影响（<0.05），在第21、31和41天均如此。在BF损伤的程度和水平以及B淋巴细胞数量方面也存在差异。根据所应用的疫苗接种方案，所检测鸡血清中抗NDV的GMT未显示出显著差异。
