Jiang Qiuruo, Sun Yichen, Gu Zhanhao, Wang Lumeng, Wu Yiqiang, Chen Jialu, Chen Zhiyi, Zheng Xiaobo, Chen Shihao
National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China.
National Engineering Research Center of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China.
Bioengineering (Basel). 2025 Jun 11;12(6):634. doi: 10.3390/bioengineering12060634.
(1) Background: The early diagnosis of keratoconus is critical for prognosis. Traditional methods like ORA and Corvis ST measure overall corneal biomechanics but lack regional specificity and are affected by intraocular pressure. In contrast, Brillouin microscopy assesses regional corneal biomechanics without such limitations; (2) Methods: In total, 25 keratoconus patients and 28 healthy controls were included in this study. Corneal biomechanics were measured using the BOSS system (Brillouin Optical Scanning System) in a 10-point mode within an 8 mm diameter, and included the mean, maximum, minimum and standard Brillouin shift. The Corvis ST parameters extracted included the CBI (Corneal Biomechanical Index), CCBI (Corvis Biomechanical Index for Chinese populations), SSI (Stress-Strain Index), DA (Deformation Amplitude), IIR (Inverse Integrated Radius), and SP-A1 (Stiffness Parameter at First Applanation); (3) Results: BOSS showed significant differences in the inferior nasal region ( = 0.004) and central region ( = 0.029) between groups, but not in peripheral regions ( = 0.781). In a comparison of the Brillouin frequency shifts measured between groups, there was no difference in the Mean ( = 0.452) and Max ( = 0.487), but the Min ( = 0.003), Standard ( = 0.000), and Max-Min ( = 0.006) all showed differences. Corvis ST identified significant differences in six parameters (CBI, CCBI, SSI, DA, IIR, and SP-A1) between groups ( < 0.001). Correlations were found between the BOSS and Corvis ST results, with moderate correlations in the inferior nasal region; (4) Conclusions: The BOSS Brillouin microscope can provide an accurate diagnostic evaluation for the corneal biomechanical differences between normal eyes and keratoconus, independent of IOP (Intraocular Pressure) and CCT (Central Corneal Thickness), with a good correlation with Corvis ST, especially in assessing regional biomechanics.
(1) 背景：圆锥角膜的早期诊断对预后至关重要。传统方法如ORA和Corvis ST可测量角膜整体生物力学，但缺乏区域特异性且受眼压影响。相比之下，布里渊显微镜评估角膜区域生物力学时不存在此类局限性；(2) 方法：本研究共纳入25例圆锥角膜患者和28例健康对照。使用BOSS系统（布里渊光学扫描系统）在直径8 mm范围内以10点模式测量角膜生物力学，包括平均、最大、最小和标准布里渊频移。提取的Corvis ST参数包括角膜生物力学指数（CBI）、中国人群Corvis生物力学指数（CCBI）、应力应变指数（SSI）、变形幅度（DA）、反向积分半径（IIR）和平坦化初始刚度参数（SP-A1）；(3) 结果：BOSS显示两组之间在下鼻侧区域（P = 0.004）和中央区域（P = 0.029）存在显著差异，但周边区域无差异（P = 0.781）。在比较两组测量的布里渊频移时，平均（P = 0.452）和最大（P = 0.487）无差异，但最小（P = 0.003）、标准差（P = 0.000）和最大-最小（P = 0.006）均显示出差异。Corvis ST确定两组之间六个参数（CBI、CCBI、SSI、DA、IIR和SP-A1）存在显著差异（P < 0.001）。发现BOSS和Corvis ST结果之间存在相关性，在下鼻侧区域具有中等相关性；(4) 结论：BOSS布里渊显微镜可独立于眼压（IOP）和中央角膜厚度（CCT），为正常眼与圆锥角膜之间的角膜生物力学差异提供准确的诊断评估，与Corvis ST具有良好的相关性，尤其在评估区域生物力学方面。
