Ravelombola Waltram, Xiong Haizheng, Bhattarai Gehendra, Manley Aurora, Cason John, Pham Hanh, Zia Bazgha, Mou Beiquan, Shi Ainong
Texas A&M AgriLife Research, 11708 Highway 70 South, Vernon, TX 76384, USA.
Department of Soil and Crop Sciences, Texas A&M University, 370 Olsen Blvd., College Station, TX 77843, USA.
Int J Mol Sci. 2025 Jun 7;26(12):5478. doi: 10.3390/ijms26125478.
Despite the fact that cowpea is one of the most drought-tolerant legumes, some genotypes with a high yield under well-watered conditions have been shown to be susceptible to drought stress, thus requiring further improvement. The objectives of this study were to conduct a genome-wide association study (GWAS) for drought tolerance in cowpea. A total of 331 cowpea genotypes were evaluated for drought tolerance. After SNP filtering, 5,884,299 SNPs were used to conduct GWAS using BLINK. The results showed: (1) a significant GWAS peak defined by a cluster of 196 significant SNPs and mapped on a 210 kb region of chromosome 5 was identified to be a good locus candidate for tolerance to trifoliate leaf chlorosis under drought stress in cowpea, (2) a strong GWAS peak was found towards the end of chromosome 1 and this peak was a good candidate locus for tolerance to unifoliate leaf chlorosis under drought stress in cowpea, and (3) a total of 25 SNPs located on chromosomes 1, 3, 5, and 11 were significantly associated with plant greenness under drought stress. This study provides a better understanding of the molecular genetics of drought tolerance in cowpea and the findings can be expanded to other crop species.
尽管豇豆是最耐旱的豆类作物之一，但一些在水分充足条件下高产的基因型已被证明对干旱胁迫敏感，因此需要进一步改良。本研究的目的是对豇豆的耐旱性进行全基因组关联研究（GWAS）。共对331个豇豆基因型进行了耐旱性评估。经过SNP筛选后，使用5,884,299个SNP通过BLINK进行GWAS。结果表明：（1）在豇豆5号染色体上一个210 kb区域内，由196个显著SNP组成的聚类所定义的一个显著GWAS峰被确定为干旱胁迫下对三出复叶萎黄病耐受性的良好候选基因座；（2）在1号染色体末端发现了一个强GWAS峰，该峰是干旱胁迫下豇豆对单叶萎黄病耐受性的良好候选基因座；（3）位于1、3、5和11号染色体上的总共25个SNP与干旱胁迫下的植株绿色度显著相关。本研究有助于更好地理解豇豆耐旱性的分子遗传学，其研究结果可推广到其他作物品种。
