Sharma Rajat, Wang Meinan, Chen Xianming, Lakkakula Indira Priyadarshini, Amand Paul St, Bernardo Amy, Bai Guihua, Bowden Robert L, Carver Brett F, Boehm Jeffrey D, Aoun Meriem
Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, USA.
Department of Plant Pathology, Washington State University, Pullman, WA, USA.
Theor Appl Genet. 2025 Mar 10;138(4):67. doi: 10.1007/s00122-025-04858-3.
The GWAS and testing with Yr gene linked markers identified 109 loci including 40 novel loci for all-stage and adult plant stage resistance to stripe rust in 459 US contemporary hard winter wheat genotypes. Stripe rust is a destructive wheat disease, caused by Puccinia striiformis f. sp. tritici (Pst). To identify sources of stripe rust resistance in US contemporary hard winter wheat, a panel of 459 Great Plains wheat genotypes was evaluated at the seedling stage against five US Pst races and at the adult plant stage in field environments in Oklahoma, Kansas, and Washington. The results showed that 7-14% of the genotypes were resistant to Pst races at the seedling stage, whereas 32-78% of genotypes were resistant at the adult plant stage across field environments, indicating the presence of adult plant resistance. Sixteen genotypes displayed a broad spectrum of resistance to all five Pst races and across all field environments. The panel was further genotyped using 9858 single-nucleotide polymorphisms (SNPs) generated from multiplex restriction amplicon sequencing (MRASeq) and the functional DNA markers linked to the known stripe rust resistance (Yr) genes Yr5, Yr15, Yr17, Yr18, Yr29, Yr36, Yr40, Yr46, and QYr.tamu-2B. A genome-wide association study (GWAS) was performed using genotypic and phenotypic data, which identified 110 SNPs and the functional markers linked to Yr15 and Yr17 to be significantly associated with stripe rust response. In addition, Yr5, Yr15, Yr17, Yr18, Yr29, and QYr.tamu-2B were detected by their functional DNA markers in the panel. This study identified 40 novel loci associated with stripe rust resistance in genomic regions not previously characterized by known Yr genes. These findings offer significant opportunities to diversify and enhance stripe rust resistance in hard winter wheat.
全基因组关联研究（GWAS）以及利用与Yr基因连锁的标记进行检测，在459个美国当代硬粒冬小麦基因型中鉴定出109个位点，其中包括40个与全生育期和成株期抗条锈病相关的新位点。条锈病是一种由条形柄锈菌小麦专化型（Pst）引起的毁灭性小麦病害。为了在美国当代硬粒冬小麦中鉴定条锈病抗性来源，对一组459个大平原小麦基因型在苗期针对5个美国Pst小种进行了评估，并在俄克拉荷马州、堪萨斯州和华盛顿州的田间环境中对成株期进行了评估。结果表明，7%-14%的基因型在苗期对Pst小种具有抗性，而在所有田间环境中，32%-78%的基因型在成株期具有抗性，表明存在成株抗性。16个基因型对所有5个Pst小种以及所有田间环境均表现出广谱抗性。利用多重限制性扩增子测序（MRASeq）产生的9858个单核苷酸多态性（SNP）以及与已知条锈病抗性（Yr）基因Yr5、Yr15、Yr17、Yr18、Yr29、Yr36、Yr40、Yr46和QYr.tamu-2B连锁的功能DNA标记，对该群体进行了进一步基因分型。利用基因型和表型数据进行了全基因组关联研究（GWAS），鉴定出110个SNP以及与Yr15和Yr17连锁的功能标记与条锈病反应显著相关。此外，通过其功能DNA标记在该群体中检测到了Yr5、Yr15、Yr17、Yr18、Yr29和QYr.tamu-2B。本研究在以前未被已知Yr基因表征的基因组区域中鉴定出40个与条锈病抗性相关的新位点。这些发现为硬粒冬小麦条锈病抗性的多样化和增强提供了重要机会。
