Nozdrenko Dmytro, Prylutskyy Yuriy, Sulyma Oleksii, Kozik Yevhenii, Vareniuk Igor, Ritter Uwe, Abramovych Tetiana, Sokolowska Inna, Maznychenko Andriy
ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Volodymyrska Str. 64, 01601 Kyiv, Ukraine.
State Institution "Institute of Traumatology and Orthopedics", National Academy of Medical Science of Ukraine, Bulvarno-Kudriavska Str. 27, 01054 Kyiv, Ukraine.
Int J Mol Sci. 2025 Jun 9;26(12):5511. doi: 10.3390/ijms26125511.
Traumatic muscle injuries often lead to prolonged functional impairments due to oxidative stress, metabolic disturbances, and structural damage. This study investigates the therapeutic potential of chronic administration of C fullerene aqueous (CFAS) solutions in enhancing muscle recovery post-injury. Rats with experimentally induced gastrocnemius muscle trauma received CFAS orally at a daily dose of 1 mg/kg body weight. Functional assessments included measurements of maximal force generation and time to peak contraction. Biochemical analyses evaluated lactate, superoxide dismutase (SOD), and catalase (CAT) levels, while histological examinations assessed muscle fiber integrity and collagen deposition. Results demonstrated significant improvements in muscle function, with a 35-40% increase in maximal force and a 27-38% acceleration in contraction time. Biochemical analysis revealed a 25% decrease in lactate concentration, potentially indicating improved metabolic function. This change is in line with normalized SOD and CAT activities, suggesting enhanced redox balance following treatment. Histological analyses revealed preserved myofibrillar architecture and reduced fibrosis in treated muscles. These findings suggest that C fullerene facilitates muscle recovery through antioxidant protection, metabolic support, and structural preservation, highlighting its potential as a therapeutic agent for muscle injuries.
创伤性肌肉损伤常因氧化应激、代谢紊乱和结构损伤而导致长期功能障碍。本研究调查了长期给予富勒烯C水基溶液（CFAS）对促进损伤后肌肉恢复的治疗潜力。实验性诱导腓肠肌损伤的大鼠每天口服剂量为1mg/kg体重的CFAS。功能评估包括最大力量产生和达到峰值收缩时间的测量。生化分析评估乳酸、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平，而组织学检查评估肌纤维完整性和胶原沉积。结果表明肌肉功能有显著改善，最大力量增加35 - 40%，收缩时间加快27 - 38%。生化分析显示乳酸浓度降低25%，这可能表明代谢功能得到改善。这一变化与SOD和CAT活性正常化一致，表明治疗后氧化还原平衡增强。组织学分析显示治疗后的肌肉中肌原纤维结构保存且纤维化减少。这些发现表明富勒烯C通过抗氧化保护、代谢支持和结构保存促进肌肉恢复，突出了其作为肌肉损伤治疗剂的潜力。
