Andreatta Gabriele, Montagnese Sara, Costa Rodolfo
Department of Biology, University of Padua, 35131 Padua, Italy.
Department of Comparative Biomedicine and Food Science, University of Padua, 35020 Padua, Italy.
Int J Mol Sci. 2025 Jun 12;26(12):5638. doi: 10.3390/ijms26125638.
Temperature is perceived primarily via transient receptor potential (TRP) channels, which are integral to the molecular machinery sensing environmental and cellular signals. Functional evidence of TRP channels' involvement in regulating cold-induced developmental/reproductive responses remains scarce. Here, we show that mutations affecting cold-sensing TRP channels antagonize the reduction in ovarian development induced by low temperatures (reproductive dormancy) in . More specifically, mutants for , , and significantly lowered dormancy levels at 12 °C and exhibited well-developed oocytes characterized by advanced vitellogenesis. Similarly, functional knockouts for , a gene encoding a phospholipase C acting downstream to Trp and Trpl, exhibited a reduced dormancy response, suggesting that Ca signaling is key to relaying cold-sensing stimuli during dormancy induction and maintenance. Finally, mutants with an altered temperature preference (i.e., exhibiting impaired cold or warm avoidance) differentially responded to the cold, either lowering or increasing dormancy levels. In summary, our phenotypic analysis provides functional evidence of developmental/reproductive modulation by specific cold-sensing TRP channels in and indicates that temperature preference affects developmental processes.
温度主要通过瞬时受体电位（TRP）通道被感知，这些通道是感知环境和细胞信号的分子机制所不可或缺的。TRP通道参与调节冷诱导的发育/生殖反应的功能证据仍然很少。在这里，我们表明，影响冷感知TRP通道的突变会拮抗低温（生殖休眠）诱导的卵巢发育减少。更具体地说，、和的突变体在12°C时显著降低了休眠水平，并表现出以卵黄发生提前为特征的发育良好的卵母细胞。同样，作为Trp和Trpl下游起作用的编码磷脂酶C的基因的功能性敲除表现出休眠反应降低，这表明钙信号是在休眠诱导和维持过程中传递冷感知刺激的关键。最后，温度偏好改变的突变体（即表现出冷或热回避受损）对寒冷有不同的反应，要么降低要么增加休眠水平。总之，我们的表型分析提供了特定冷感知TRP通道在中对发育/生殖进行调节的功能证据，并表明温度偏好会影响发育过程。
