Xie Xiang, Yang Zhonglong, Zhong Wei, Li Hongjiang, Deng Wenjing, Ruan Ying, Liu Chunlin
Key Laboratory of Hunan Provincial on Crop Epigenetic Regulation and Development, Hunan Agricultural University, Changsha 410128, China.
Yuelushan Laboratory, Hunan Agricultural University, Changsha 410128, China.
Int J Mol Sci. 2025 Jun 13;26(12):5678. doi: 10.3390/ijms26125678.
Rapeseed () is an essential oil resource, but its yield can be significantly compromised by () infection. Due to the absence of rapeseed strains that are highly or completely immune to , enhancing rapeseed resistance through genetic approaches is challenging. In this study, we developed a novel method to enhance rapeseed resistance to using β-ocimene. Our results demonstrated that β-ocimene treatment significantly strengthened the defense capabilities of rapeseed. β-ocimene treatment can simultaneously activate multiple defense-related signaling pathways, including jasmonic acid signaling, salicylic acid signaling, and MAPK signaling, in rapeseed, while also inducing the accumulation of secondary metabolites coniferyl aldehyde-a key secondary metabolite in the phenylpropanoid pathway critical for plant defense responses. Furthermore, applying coniferyl aldehyde to the leaves of rapeseed can remarkably enhance its resistance to sclerotinia disease. Collectively, these findings confirm that β-ocimene activates the defense system of rapeseed, elevates the content of coniferyl aldehyde, and thereby enables rapeseed to effectively combat sclerotinia disease. The metabolomics data are available via MetaboLights under the identifier MTBLS12510. In conclusion, this study not only uncovers the mechanism by which β-ocimene induces rapeseed resistance to sclerotinia disease but also presents a novel approach for its prevention and control.
油菜籽是一种重要的油脂资源，但其产量会因核盘菌感染而显著受损。由于缺乏对核盘菌具有高度免疫或完全免疫的油菜品种，通过基因方法增强油菜抗性具有挑战性。在本研究中，我们开发了一种利用β-罗勒烯增强油菜对核盘菌抗性的新方法。我们的结果表明，β-罗勒烯处理显著增强了油菜的防御能力。β-罗勒烯处理可同时激活油菜中多种与防御相关的信号通路，包括茉莉酸信号通路、水杨酸信号通路和丝裂原活化蛋白激酶（MAPK）信号通路，同时还诱导次生代谢产物松柏醛的积累，松柏醛是苯丙烷类途径中的一种关键次生代谢产物，对植物防御反应至关重要。此外，将松柏醛施用于油菜叶片可显著增强其对菌核病的抗性。总的来说，这些发现证实β-罗勒烯激活了油菜的防御系统，提高了松柏醛的含量，从而使油菜能够有效抵抗菌核病。代谢组学数据可通过MetaboLights获取，标识符为MTBLS12510。总之，本研究不仅揭示了β-罗勒烯诱导油菜对菌核病抗性的机制，还提出了一种新的防治方法。
