Chung Kyung Bae, Lee Young In, Kim Jihee, Nguyen Ngoc Ha, Kim Yoo Jin, Jung Inhee, Lee Jeonghoon, Jeon Hyun-Jeong, Lim Youbong, Lee Sung Jun, Lee Ju Hee
Department of Dermatology, Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Scar Laser and Plastic Surgery Center, Yonsei Cancer Hospital, Yonsei University College of Medicine, Seoul 03722, Republic of Korea.
Int J Mol Sci. 2025 Jun 18;26(12):5854. doi: 10.3390/ijms26125854.
Plasma, an ionized gas composed of charged particles, has shown therapeutic potential in enhancing biological processes such as wound healing and tissue integration. Implants, such as silicone and human acellular dermal matrix (hADM), are commonly used in reconstructive surgery, but improving their biocompatibility and integration remains a challenge. This study investigated the effects of vacuum plasma treatment on silicone and hADM implants using an in vivo rat model. Plasma-treated and untreated implants were inserted subcutaneously, and tissue samples were collected at 1, 4, and 8 weeks post-implantation. Histological and immunohistochemical analyses were performed to assess inflammation, cellular infiltration, collagen formation (neocollagenesis), and angiogenesis. Results showed that plasma-treated silicone and hADM implants had significantly reduced capsule thickness at weeks 4 and 8 compared to untreated controls, indicating a lower chronic inflammatory response. Plasma treatment also promoted greater fibroblast infiltration and enhanced neocollagenesis within the hADM implants. Furthermore, immunohistochemical staining revealed a notable increase in blood vessel formation around and within the plasma-treated hADM implants, suggesting improved vascularization. In conclusion, vacuum plasma treatment enhances the biocompatibility and tissue integration of implants by reducing inflammation and promoting cellular and vascular responses, offering promising potential for improving outcomes in reconstructive surgery.
等离子体是一种由带电粒子组成的电离气体，已显示出在促进伤口愈合和组织整合等生物过程方面的治疗潜力。植入物，如硅胶和人脱细胞真皮基质（hADM），常用于重建手术，但提高它们的生物相容性和整合性仍然是一项挑战。本研究使用体内大鼠模型研究了真空等离子体处理对硅胶和hADM植入物的影响。将经等离子体处理和未经处理的植入物皮下植入，在植入后1周、4周和8周收集组织样本。进行组织学和免疫组织化学分析以评估炎症、细胞浸润、胶原形成（新胶原形成）和血管生成。结果表明，与未处理的对照组相比，经等离子体处理的硅胶和hADM植入物在第4周和第8周时包膜厚度显著降低，表明慢性炎症反应较低。等离子体处理还促进了hADM植入物内成纤维细胞的更多浸润并增强了新胶原形成。此外，免疫组织化学染色显示，经等离子体处理的hADM植入物周围和内部的血管形成显著增加，表明血管化得到改善。总之，真空等离子体处理通过减少炎症并促进细胞和血管反应来增强植入物的生物相容性和组织整合性，为改善重建手术的效果提供了有前景的潜力。
