Van Winkle L J, Campione A L, Kester S E
J Exp Zool. 1985 Jul;235(1):141-5. doi: 10.1002/jez.1402350117.
Two- and four-cell mouse embryos exhibited both Na+-dependent and Na+-independent components of zwitterionic alpha-amino acid transport, which we tentatively ascribe to the A and L amino acid transport systems, respectively. Uptake of taurine was virtually all Na+-dependent and is probably via the beta system. Na+-independent L-lysine uptake by two-cell embryos may have been via system y+. The small amount of lysine transport which was Na+-dependent (30% of the total) could not be attributed to any well known transport system and may have been due to the early ontogenetic expression of a newly described transport system which predominates in preimplantation blastocysts. We conclude that the rate of Na+-dependent amino acid transport in two-cell mouse embryos could be significantly affected in situ by changes in the [Na+] which are known to occur in oviductal fluid.
二细胞和四细胞小鼠胚胎表现出两性离子α-氨基酸转运的钠离子依赖性和非钠离子依赖性成分，我们分别初步将其归因于A和L氨基酸转运系统。牛磺酸的摄取几乎完全依赖钠离子，可能是通过β系统。二细胞胚胎对非钠离子依赖性L-赖氨酸的摄取可能是通过y+系统。少量依赖钠离子的赖氨酸转运（占总量的30%）不能归因于任何已知的转运系统，可能是由于一种新描述的转运系统在植入前胚泡中占主导地位的早期个体发育表达。我们得出结论，已知输卵管液中[Na+]的变化可原位显著影响二细胞小鼠胚胎中依赖钠离子的氨基酸转运速率。
