Xie Xiaohu, Zhuang Dingding, Li Longhui, Wu Tingting, Shen Wenwen, Liu Yue, Xu Wenjin, Hong Qingxiao, Xu Zemin, Chen Weisheng, Gu Jun, Zhou Wenhua, Liu Huifen
Department of Psychiatry, Affiliated Kangning Hospital of Ningbo University, Ningbo, China.
Key Laboratory of Addiction Research of Zhejiang Province, Ningbo, China.
Psychiatry Investig. 2025 Jun;22(6):730-735. doi: 10.30773/pi.2025.0090. Epub 2025 Jun 16.
N-methyl-D-aspartate (NMDA) receptors are involved in the development of opioid use disorder. The (GT)n polymorphism (rs3219790) in the NMDA receptor 2A subunit gene (GRIN2A) has been proposed as a potential biomarker for risk of opioid use disorder. In this case-control study, we investigated the association between rs3219790 and opioid use disorder in a Chinese Han population.
A total of 538 heroin dependent patients and 400 healthy controls were recruited. The genotypes of (GT)n repeats were determined using a polymerase chain reaction-amplifying fragment length polymorphism assay. The association of the (GT)n polymorphism with opioid use disorder and cravings was assessed.
The frequency of the (GT)26 allele in patients with opioid use disorder was significantly greater than that in the controls (p=0.029, odds ratio=1.264, 95% confidence interval=1.025-1.560), consistent with previous findings. Compared with homozygous carriers of short alleles, carriers of long alleles demonstrated significantly stronger drug cravings (p<0.05).
The results confirm that the (GT)26 allele of rs3219790 in the GRIN2A promoter is associated with opioid use disorder. Additionally, a longer rs3219790 allele is correlated with stronger drug cravings.
N-甲基-D-天冬氨酸（NMDA）受体参与阿片类物质使用障碍的发展。NMDA受体2A亚基基因（GRIN2A）中的（GT）n多态性（rs3219790）已被提出作为阿片类物质使用障碍风险的潜在生物标志物。在这项病例对照研究中，我们调查了rs3219790与中国汉族人群中阿片类物质使用障碍之间的关联。
共招募了538名海洛因依赖患者和400名健康对照。使用聚合酶链反应-扩增片段长度多态性分析确定（GT）n重复的基因型。评估（GT）n多态性与阿片类物质使用障碍及渴求之间的关联。
阿片类物质使用障碍患者中（GT）26等位基因的频率显著高于对照组（p = 0.029，优势比 = 1.264，95%置信区间 = 1.025 - 1.560），与先前的研究结果一致。与短等位基因纯合携带者相比，长等位基因携带者表现出显著更强的药物渴求（p < 0.05）。
结果证实GRIN2A启动子中rs3219790的（GT）26等位基因与阿片类物质使用障碍相关。此外，较长的rs3219790等位基因与更强的药物渴求相关。
