来自专性厌氧细菌盲肠罗氏菌的鞭毛的纯化与特性分析

Purification and characterization of flagella from Roseburia cecicola, an obligately anaerobic bacterium.

作者信息

Martin J H, Savage D C

出版信息

J Gen Microbiol. 1985 Aug;131(8):2075-8. doi: 10.1099/00221287-131-8-2075.

DOI:10.1099/00221287-131-8-2075

Abstract

Flagella from Roseburia cecicola, an obligately anaerobic bacterium originally isolated from murine caecal mucosa, were purified by mechanical shearing followed by differential centrifugation. Purity of the flagellar preparation was determined by polyacrylamide gel electrophoresis, electron microscopy and chemical analysis. The flagella were composed of a single protein subunit (flagellin) with an estimated molecular weight of 42 000. The amino acid composition of the flagellin was similar to that of some facultatively anaerobic and aerobic bacteria.

摘要

来自盲肠罗斯氏菌（一种最初从小鼠盲肠黏膜分离出的专性厌氧菌）的鞭毛，通过机械剪切后进行差速离心进行纯化。通过聚丙烯酰胺凝胶电泳、电子显微镜和化学分析来确定鞭毛制剂的纯度。这些鞭毛由单一蛋白质亚基（鞭毛蛋白）组成，估计分子量为42000。鞭毛蛋白的氨基酸组成与一些兼性厌氧和好氧细菌的相似。