Shapiro L, Maizel J V
J Bacteriol. 1973 Jan;113(1):478-85. doi: 10.1128/jb.113.1.478-485.1973.
During the normal cell cycle of Caulobacter crescentus, flagella are released into the culture fluid as swarmer cells differentiate into stalked cells. The released flagellum is composed of a filament, hook, and rod. The molecular weight of purified flagellin (subunit of flagella filament) is 25,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The formation of a flagellum opposite the stalk has been observed by microscope during the differentiation of a stalked cell in preparation for cell division. By pulsing synchronized cultures with (14)C-amino acids it has been demonstrated that the synthesis of flagellin occurs approximately 30 to 40 min before cell division. Flagellin, therefore, is synthesized at a discrete time in the cell cycle and is assembled into flagella at a specific site on the cell. A mutant of C. crescentus that fails to synthesize flagellin has been isolated.
在新月柄杆菌的正常细胞周期中，随着游动细胞分化为柄细胞，鞭毛会释放到培养液中。释放出的鞭毛由细丝、钩和杆组成。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳测定，纯化鞭毛蛋白（鞭毛细丝的亚基）的分子量为25,000。在柄细胞为细胞分裂做准备的分化过程中，通过显微镜观察到在与柄相对的位置形成了一根鞭毛。通过用（14）C - 氨基酸脉冲同步培养物，已证明鞭毛蛋白的合成大约在细胞分裂前30至40分钟发生。因此，鞭毛蛋白在细胞周期的特定时间合成，并在细胞的特定部位组装成鞭毛。已分离出一种不能合成鞭毛蛋白的新月柄杆菌突变体。
