Shatova O P, Shestopalov A V, Zlatnik E Yu, Novikova I A, Goncharova A S, Maksimov A Yu
N.I. Pirogov Russian National Research Medical University, Moscow, Russia.
National Medical Research Centre for Oncology, Rostov-on-Don, Russia.
Biomed Khim. 2025 Jun;71(3):209-216. doi: 10.18097/PBMCR1554.
Gut microbiota is one of the key suppliers of tryptophan metabolites, which perform various functions in the host organism, including their role as signaling molecules. Fecal microbiota transplantation (FMT) is widely used as a method for determining the contribution of microorganisms to the content of various metabolites in the holoorganism. In this regard, the aim of our study was to investigate the effect of FMT on the level of tryptophan metabolites in feces and blood in gnotobiotic mice. It was found that both before and after FMT, indole-3-lactate, and quinolinic acid were the dominant tryptophan metabolites in the intestine. FMT increased the content of both indoles (indole-3-acetate, indole-3-acrylate, indole-3-butyrate, indole-3-lactate) and kynurenines (anthranilic and xanthurenic acids) in the intestine. In serum of mice after FMT, indole metabolites (indole-3-butyrate, indole-3-carboxaldehyde, indole-3-lactate, indole-3-propionate) predominantly increased; however, tryptamine and xanthurenic acid also demonstrated a clear increase. The use of FMT demonstrates that the intestinal microbiota is a source of not only indole derivatives of tryptophan, but also metabolites of the kynurenine pathway.
肠道微生物群是色氨酸代谢产物的主要供应者之一，这些代谢产物在宿主生物体中发挥着各种功能，包括作为信号分子的作用。粪便微生物群移植（FMT）被广泛用作一种确定微生物对全生物体中各种代谢产物含量贡献的方法。在这方面，我们研究的目的是调查FMT对无菌小鼠粪便和血液中色氨酸代谢产物水平的影响。结果发现，在FMT前后，吲哚 - 3 - 乳酸和喹啉酸都是肠道中主要的色氨酸代谢产物。FMT增加了肠道中吲哚（吲哚 - 3 - 乙酸、吲哚 - 3 - 丙烯酸、吲哚 - 3 - 丁酸、吲哚 - 3 - 乳酸）和犬尿氨酸（邻氨基苯甲酸和黄尿酸）的含量。在FMT后的小鼠血清中，吲哚代谢产物（吲哚 - 3 - 丁酸、吲哚 - 3 - 甲醛、吲哚 - 3 - 乳酸、吲哚 - 3 - 丙酸）主要增加；然而，色胺和黄尿酸也有明显增加。FMT的应用表明，肠道微生物群不仅是色氨酸吲哚衍生物的来源，也是犬尿氨酸途径代谢产物的来源。
