Thompson S N, Lee R W
J Parasitol. 1985 Oct;71(5):652-61.
31P nuclear magnetic resonance spectroscopy was applied successfully to whole intact and de-shelled snails and to the isolated digestive gland-gonad tissue complex as well as other tissues of Biomphalaria glabrata. Several phosphorus metabolites, including ATP and ADP, were observed. The mean ATP/ADP ratio calculated for the tissue complex was 3.1 and the ATP concentration was 0.73 nmoles/mg tissue fresh weight. Assignments for AMP, sugar phosphates, and inorganic phosphate peaks were tentatively made. A major phosphorus component was identified as a phosphonate and this metabolite was also present in egg masses and the albumin gland. Phosphoarginine was not observed in the tissue complex but was present in whole animals. Infection by Schistosoma mansoni resulted in marked alteration in the relative levels of phosphorus metabolites in the digestive gland-gonad complex during the course of infection. The decrease in phosphonate was particularly notable. The relative level of a metabolite occurring at -1.1 ppm was also decreased but its identity remained unknown. The ATP/ADP ratio was not affected by infection, but an increase in the relative level of inorganic phosphate suggested a possible decrease in phosphorylation potential.
31P核磁共振光谱法已成功应用于完整的和去壳的福寿螺以及分离出的消化腺-性腺组织复合体和光滑双脐螺的其他组织。观察到了几种磷代谢物，包括ATP和ADP。计算得出的组织复合体的平均ATP/ADP比率为3.1，ATP浓度为0.73纳摩尔/毫克组织鲜重。对AMP、磷酸糖和无机磷酸峰进行了初步归属。一种主要的磷成分被鉴定为膦酸盐，这种代谢物也存在于卵块和蛋白腺中。在组织复合体中未观察到磷酸精氨酸，但在整个动物体内存在。曼氏血吸虫感染导致感染过程中消化腺-性腺复合体中磷代谢物的相对水平发生显著变化。膦酸盐的减少尤为明显。在-1.1 ppm处出现的一种代谢物的相对水平也降低了，但其身份仍然未知。ATP/ADP比率不受感染影响，但无机磷酸相对水平的增加表明磷酸化潜力可能降低。
