Jiao Siqi, Shen Zhiqiang, Fang Qiongya, Liu Xiaorui, Hao Yongkun, Kong Yidi, Peng Sibo, Li Min, Wang Guiqin
Joint Laboratory of Modern Agricultural Technology International Cooperation, Ministry of Education, Key Laboratory of Animal Production, Product Quality and Security, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China.
Jilin Academy of Fishery Sciences, Changchun 130033, China.
Aquat Toxicol. 2025 Sep;286:107450. doi: 10.1016/j.aquatox.2025.107450. Epub 2025 Jun 19.
This study examined the impact of Polystyrene microplastics (PS-MPs) on inflammation, apoptosis and autophagy within the liver, intestine, kidney, and gill of northern snakehead (Channa argus). In this study, 600 snakeheads (mean body weight: 10.5 ± 0.5 g) were randomly divided into four groups, each with three replicates. These groups were administered diets with incremental concentrations of PS (0 (CON), 500 (PS500), 1000 (PS1000), and 2000 ng/L (PS2000)) over a duration of four weeks. Histopathological analysis of the liver, intestine, kidney, and gill showed different degrees of pathological changes in the PS-added groups. Exposure to PS significantly increased ROS levels and elevated MDA content, while simultaneously reducing the activity of antioxidant enzymes (CAT, GSH, SOD, and GSH-PX) in these organs. The immune parameters (C3, C4, LYS, and IgM) in the liver, kidney, intestine, and gills of C. argus exposed to varying PS also changes. Additionally, increases in PS levels were accompanied by decreased expression of genes associated with antioxidant functions (cat, sod, gsh-px, nqo-1, ho-1, and gst). Analysis via quantitative real-time PCR (qRT-PCR) revealed changes in the mRNA levels of genes related to inflammation (tnf-α, il-8, nf-κb, jnk, il-1β, il-12, myd88, il-10 and iκbα), apoptosis (p53, bad, csa-9, cyt-c, bax, fas-2, and apaf-1), and autophagy (p62, atg5, lc-3, and beclin-1) in the liver, intestine, kidney, and gill. In summary, current research indicates that microplastics in water damage the antioxidant defense system, induce cell apoptosis, enhance inflammation, and lead to tissue damage. The adverse effects of microplastics on tissues may be due to TNF-α/ NF-κB/ Beclin-1 signaling pathways.
本研究考察了聚苯乙烯微塑料（PS - MPs）对乌鳢（Channa argus）肝脏、肠道、肾脏和鳃中炎症、细胞凋亡和自噬的影响。在本研究中，600条乌鳢（平均体重：10.5±0.5克）被随机分为四组，每组三个重复。这些组在四周的时间内被投喂含有递增浓度PS（0（对照组）、500（PS500）、1000（PS1000）和2000纳克/升（PS2000））的饲料。对肝脏、肠道、肾脏和鳃的组织病理学分析显示，添加PS的组出现了不同程度的病理变化。暴露于PS显著提高了活性氧水平并升高了丙二醛含量，同时降低了这些器官中抗氧化酶（CAT、GSH、SOD和GSH - PX）的活性。暴露于不同PS水平的乌鳢肝脏、肾脏、肠道和鳃中的免疫参数（C3、C4、LYS和IgM）也发生了变化。此外，PS水平的升高伴随着与抗氧化功能相关基因（cat、sod、gsh - px、nqo - 1、ho - 1和gst）表达的降低。通过定量实时PCR（qRT - PCR）分析发现，肝脏、肠道、肾脏和鳃中与炎症（tnf -α、il - 8、nf -κb、jnk、il - 1β、il - 12、myd88、il - 10和iκbα）、细胞凋亡（p53、bad、csa - 9、cyt - c、bax、fas - 2和apaf - 1）以及自噬（p62、atg5、lc - 3和beclin - 1）相关的基因的mRNA水平发生了变化。总之，当前研究表明，水中的微塑料会损害抗氧化防御系统，诱导细胞凋亡，增强炎症反应，并导致组织损伤。微塑料对组织的不利影响可能归因于TNF -α/ NF -κB/ Beclin - 1信号通路。
