Bauer A J, Reed J B, Sanders K M
J Physiol. 1985 Sep;366:221-32. doi: 10.1113/jphysiol.1985.sp015793.
A cross-sectional preparation was designed in which multiple micro-electrodes can be precisely positioned to impale smooth muscle cells anywhere from the serosa to the submucosa. Intracellular electrical recordings were obtained from gastric antral circular muscle cells from the myenteric plexus to the submucosa. The resting membrane potential changed linearly as a function of distance from the myenteric plexus to the submucosa. Slow wave upstroke dV/dt, upstroke potential amplitude, and plateau potential amplitude changed linearly as a function of distance from the myenteric plexus to the submucosa. When slow waves were recorded simultaneously from a circular cell near the myenteric plexus and from a cell near the submucosa, the event always occurred first in the cell near the myenteric plexus. Electrical differences did not appear to be caused by electrotonic decay of slow waves as they propagated through the circular muscle. Electrical differences could not be explained on the basis of differences in intrinsic neural activity or prostaglandin synthesis. Membrane polarization could not explain the differences in slow waves between myenteric and submucosal circular muscle cells. The conclusion of this paper is that fundamental differences exist between the excitability mechanisms and/or passive membrane properties of cells near the myenteric plexus and the submucosa. These differences might be manifest in different motor performance of these two muscle cell populations.
设计了一种横断面标本，其中多个微电极可精确放置，以刺入从浆膜到粘膜下层任何位置的平滑肌细胞。从胃窦环形肌细胞（从肌间神经丛到粘膜下层）获取细胞内电记录。静息膜电位随从肌间神经丛到粘膜下层的距离呈线性变化。慢波上升速率dV/dt、上升电位幅度和平台电位幅度随从肌间神经丛到粘膜下层的距离呈线性变化。当同时从肌间神经丛附近的环形细胞和粘膜下层附近的细胞记录慢波时，该事件总是首先发生在肌间神经丛附近的细胞中。电差异似乎不是由慢波在环形肌中传播时的电紧张性衰减引起的。电差异不能基于内在神经活动或前列腺素合成的差异来解释。膜极化不能解释肌间神经丛和粘膜下层环形肌细胞之间慢波的差异。本文的结论是，肌间神经丛附近和粘膜下层的细胞在兴奋性机制和/或被动膜特性方面存在根本差异。这些差异可能在这两种肌肉细胞群的不同运动表现中体现出来。
