Wang Gen, Zhu Xiaodong, Liang Shaoshan, Xu Feng, Liang Dandan, Zeng Caihong
National Clinical Research Center for Kidney Diseases, Jinling Hospital, Affiliated Hospital of Medical School, Nanjing University, Nanjing, China.
Ren Fail. 2025 Dec;47(1):2522325. doi: 10.1080/0886022X.2025.2522325. Epub 2025 Jun 26.
Electron microscopic examination of renal biopsies is crucial for the diagnosis of kidney disease. In this study, we present an optimized protocol that significantly reduces processing time to 19.5 h, compared to 120 h with conventional methods, while preserving ultrastructural quality in 55 renal biopsy specimens. The modified method incorporates the use of higher concentrations of fixatives, shortened fixation duration, low-viscosity resin embedding, and the application of uncoated copper grids. Comparative analysis of renal specimens processed using conventional and optimized methods demonstrated equivalent preservation of critical ultrastructural features, including the glomerular basement membranes, podocytes and tubular epithelium. The total processing time was markedly reduced to 19.5 h, compared to several days with conventional methods. This rapid protocol enables timely ultrastructural evaluation of renal biopsies to facilitate prompt diagnosis and decision-making, highlighting the significance of optimizing diagnostic pathology methodologies for enhancing efficiency and maintaining accuracy.
肾活检的电子显微镜检查对肾脏疾病的诊断至关重要。在本研究中，我们提出了一种优化方案，与传统方法所需的120小时相比，该方案可将处理时间显著缩短至19.5小时，同时在55份肾活检标本中保持超微结构质量。改良方法包括使用更高浓度的固定剂、缩短固定时间、低粘度树脂包埋以及应用未镀膜的铜网。对采用传统方法和优化方法处理的肾脏标本进行的对比分析表明，关键超微结构特征（包括肾小球基底膜、足细胞和肾小管上皮）的保存情况相当。与传统方法需要数天相比，总处理时间显著缩短至19.5小时。这种快速方案能够及时对肾活检进行超微结构评估，以促进快速诊断和决策，凸显了优化诊断病理方法对于提高效率和保持准确性的重要性。
